Ubezio P
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Comput Methods Programs Biomed. 1990 Mar-Apr;31(3-4):255-66. doi: 10.1016/0169-2607(90)90010-7.
A program has been implemented on a VAX computer that simulates the progression of cells through the cell cycle and generates data similar to those obtained in flow cytometry with different techniques. Features of the program are general applicability and flexibility, options including consideration of (a) mean duration of cell cycle phases, (b) their inter-cell distribution, (c) a first order commitment from G0 into G1 phase, and (d) a total or partial block of the output from any phase. Examples are given of simulated flow cytometric experiments of drug-induced cell cycle perturbation and of bromodeoxyuridine pulse labeling. This program should help to acquire a correct understanding of the relationship between kinetic features and flow cytometric data.
已在VAX计算机上实现了一个程序，该程序可模拟细胞在细胞周期中的进程，并生成与通过不同技术进行流式细胞术获得的数据相似的数据。该程序的特点是具有普遍适用性和灵活性，其选项包括考虑（a）细胞周期各阶段的平均持续时间，（b）它们在细胞间的分布，（c）从G0期到G1期的一级承诺，以及（d）任何阶段输出的全部或部分阻滞。给出了药物诱导的细胞周期扰动和溴脱氧尿苷脉冲标记的模拟流式细胞术实验的示例。该程序应有助于正确理解动力学特征与流式细胞术数据之间的关系。
