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冷冻干预以减少从鸡肝中分离出的弯曲杆菌数量。

Freezing as an intervention to reduce the numbers of campylobacters isolated from chicken livers.

机构信息

School of Veterinary Sciences, University of Bristol, Langford, Bristol, UK.

出版信息

Lett Appl Microbiol. 2013 Sep;57(3):206-13. doi: 10.1111/lam.12098. Epub 2013 May 28.

Abstract

The aims of this study were (i) to determine the prevalence and numbers of campylobacters in 63 samples of raw livers purchased at retail across the UK and (ii) to investigate whether the freezing of chicken livers contaminated with Campylobacter was a reliable method for decontamination. Chicken livers naturally contaminated with campylobacters were subjected to freezing at -15 and -25°C for one day and 7 days. Numbers of campylobacters on the livers were determined immediately before and after a 24-h or 7-days freeze treatment and daily during 3 days post-thaw refrigerated storage. Freezing for 24 h at -25°C can reduce numbers of Campylobacter by up to 2 log10 CFU g(-1). Freezing the livers for 24 h at -25°C, thawing overnight in a fridge set to 4°C and refreezing for another 24 h at -25°C reduced the numbers of campylobacters by up to three logs. Reduction in the numbers of campylobacters was significantly greater following a second freeze treatment compared with a single freeze treatment.

摘要

本研究的目的是

(i)确定在英国各地零售的 63 份生肝脏样本中弯曲杆菌的流行率和数量;(ii)研究冷冻受弯曲杆菌污染的鸡肝是否是一种可靠的消毒方法。受弯曲杆菌自然污染的鸡肝在-15 和-25°C 下冷冻 1 天和 7 天。在 24 小时或 7 天的冷冻处理前后以及解冻后冷藏储存的 3 天内,每天测定肝脏上的弯曲杆菌数量。在-25°C 下冷冻 24 小时可使弯曲杆菌数量减少 2 个对数 10CFU g(-1)。将肝脏在-25°C 下冷冻 24 小时,在 4°C 的冰箱中过夜解冻,然后再在-25°C 下冷冻 24 小时,可使弯曲杆菌数量减少 3 个对数。与单次冷冻处理相比,第二次冷冻处理可显著降低弯曲杆菌数量。

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