School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia.
Anal Chem. 2013 Jun 4;85(11):5475-82. doi: 10.1021/ac4005875. Epub 2013 May 21.
A new phylogenetics approach and algorithm with which to chart the evolutionary history of organisms is presented. It utilizes mass spectral data produced from the proteolytic digestion of proteins, rather than partial or complete gene or translated gene sequences. The concept and validity of the approach is demonstrated herein using both theoretical and experimental mass data, together with the translated gene sequences of the hemagglutinin protein of the influenza virus. A comparison of the mass trees with conventional sequenced-based phylogenetic trees, using two separate tree comparison algorithms, reveals a high degree of similarity and congruence among the trees. Given that the mass map data can be generated more rapidly than gene sequences, even when next generation parallel sequencing is employed, mass trees offer new opportunities and advantages for phylogenetic analysis.
提出了一种新的系统发生学方法和算法，用于绘制生物体的进化历史。它利用从蛋白质的蛋白水解消化产生的质谱数据，而不是部分或完整的基因或翻译基因序列。本文通过使用理论和实验质量数据以及流感病毒血凝蛋白的翻译基因序列，演示了该方法的概念和有效性。使用两种不同的树比较算法，将质量树与传统基于测序的系统发育树进行比较，揭示了树之间具有高度的相似性和一致性。鉴于质量图谱数据的生成速度可以比基因序列更快，即使使用下一代并行测序也是如此，因此质量树为系统发育分析提供了新的机会和优势。
