Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 2R2, Canada.
Can J Microbiol. 2013 May;59(5):359-61. doi: 10.1139/cjm-2013-0040. Epub 2013 Mar 27.
A real-time polymerase chain reaction assay that uses degenerate primers and a dual-labelled probe was developed to detect the bexA gene of Haemophilus influenzae, including those belonging to non-b serotypes as well as clonal division II strains. This assay is sensitive and specific, detecting 20 copies of the gene, but negative with a variety of bacteria associated with meningitis and bacteremia or septicemia.
建立了一种实时聚合酶链反应检测方法，使用简并引物和双标记探针，用于检测流感嗜血杆菌的 bexA 基因，包括非 b 血清型和克隆型 II 株。该检测方法具有较高的灵敏度和特异性，可检测到 20 个拷贝的基因，但与各种与脑膜炎和菌血症或败血症相关的细菌无交叉反应。
