Flemish Institute for Technological Research (VITO), Unit Environmental Analysis and Technology, Boeretang 200, 2400 Mol, Belgium.
J Chromatogr A. 2013 Jun 14;1294:25-32. doi: 10.1016/j.chroma.2013.03.054. Epub 2013 Mar 25.
Phthalates, which are ubiquitous in the environment, are readily metabolized in human bodies to their respective monoesters. These phthalate monoesters are non-persistent with short half-lives, which make them the ideal biomarkers of human exposure to phthalates. In this study a direct analysis method without preconcentration was developed and validated for the following phthalate ester metabolites in urine: mono-(2-ethylhexyl) phthalate, mono-(2-ethyl-5-hydroxyhexyl) phthalate, mono-(2-ethyl-5-oxohexyl)phthalate, monobenzyl phthalate, mono-isobutylphthalate, mono-n-butyl phthalate and monoethyl phthalate. The recovery of the phthalate ester metabolites varied between 97% and 104%. The intraday precision for the replicate analysis (n=10) of a urine sample did not exceed 5% for most of the compounds. The coefficient of variance amounted to 2-3%. The limit of quantification was set equal to 0.5μg/L for the majority of the compounds. A comparison between the direct analysis method and a foregoing solid phase extraction (SPE) of the urine sample was made. Finally, the applicability of the direct analysis method was tested in three interlaboratory comparisons.
邻苯二甲酸酯在环境中普遍存在，容易在人体中代谢为各自的单酯。这些邻苯二甲酸单酯具有非持久性和短半衰期的特点，使其成为人体暴露于邻苯二甲酸酯的理想生物标志物。在这项研究中，开发并验证了一种无需预浓缩的直接分析方法，用于检测尿液中的以下邻苯二甲酸酯代谢物：邻苯二甲酸二（2-乙基己基）酯、邻苯二甲酸二（2-乙基-5-羟基己基）酯、邻苯二甲酸二（2-乙基-5-氧代己基）酯、邻苯二甲酸单苄基酯、邻苯二甲酸异丁酯、邻苯二甲酸正丁酯和邻苯二甲酸乙酯。邻苯二甲酸酯代谢物的回收率在 97%至 104%之间。对于大多数化合物，尿液样品的重复分析（n=10）日内精密度不超过 5%。变异系数达到 2-3%。大多数化合物的定量限设定为 0.5μg/L。比较了直接分析方法和先前的尿液固相萃取（SPE）方法。最后，在三个实验室间比较中测试了直接分析方法的适用性。
