胰岛素样生长因子 1 受体在大鼠视神经损伤后的视网膜表达。
Expression of insulin-like growth factor 1 receptor in rat retina following optic nerve injury.
机构信息
Department of Ophthalmology, The Second Affiliated Hospital of Soochow University, Suzhou, China.
出版信息
Acta Ophthalmol. 2013 Sep;91(6):e427-31. doi: 10.1111/aos.12096. Epub 2013 May 7.
PURPOSE
To investigate the apoptosis in retinal ganglion cells (RGCs) and insulin-like growth factor 1 receptor (IGF-1R) in the retina following optic nerve crush.
METHODS
Healthy Wistar rats (N = 70) were divided into two groups: a normal control group and an optic nerve injury group. Immunohistochemistry and flow cytometry were performed to detect the expression of IGF-1R and to measure the apoptosis of RGCs, respectively.
RESULTS
Immunohistochemistry revealed that at 1 hr after optic nerve injury, IGF-1R immunoreactivity began to increase and reached a maximal level at 24 hr (p < 0.05), where it remained elevated up to 14 days after injury. RGC apoptosis in the normal control group was 0.53%, while the apoptosis rate in the optic nerve injury group increased over time. The apoptosis rate in the optic nerve injury group was 1.4% at 1 hr, 4.4% at 6 hr, 5.2% at 12 hr and reached a maximal level (8.5%) at 24 hr. Subsequently, the rate declined to 1.9% 7 days after injury and 0.9% 2 weeks after injury.
CONCLUSION
The IGF-1R immunereactivity in the retina increased after optic nerve injury. IGF-1R may regulate the apoptosis and regeneration of RGCs at different stages after optic nerve injury.
目的
探讨视神经挤压后视网膜神经节细胞（RGC）凋亡和胰岛素样生长因子 1 受体（IGF-1R）的变化。
方法
健康 Wistar 大鼠（N=70）分为正常对照组和视神经损伤组。采用免疫组化和流式细胞术分别检测 IGF-1R 的表达和 RGC 的凋亡。
结果
视神经损伤后 1 h，IGF-1R 免疫反应开始增强，24 h 达到高峰（p＜0.05），直至损伤后 14 天仍保持升高。正常对照组 RGC 凋亡率为 0.53%，视神经损伤组凋亡率随时间逐渐增加。视神经损伤后 1 h 为 1.4%，6 h 为 4.4%，12 h 为 5.2%，24 h 达到高峰（8.5%）。随后，7 d 时降至 1.9%，2 周时降至 0.9%。
结论
视神经损伤后视网膜 IGF-1R 免疫反应增强。IGF-1R 可能在视神经损伤后不同时期调节 RGC 的凋亡和再生。
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