Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, School of Chemistry & Chemical Engineering, Huaiyin Normal University, Huaian 223300, PR China.
Biosens Bioelectron. 2013 Oct 15;48:56-60. doi: 10.1016/j.bios.2013.03.081. Epub 2013 Apr 11.
In this paper, a nonenzymatic glucose biosensor based on perovskite LaTiO3-Ag0.2(LTA) modified electrode was presented. The morphology and the composition of the perovskite LaTiO₃-Ag0.2 nanomaterials were characterized by using scanning electron microscopy (SEM) and X-ray diffraction (XRD) respectively. The LaTiO₃-Ag0.2(LTA) composite was investigated by electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, CV and chronoamperometry (I-t) study revealed that, compared with the bare glassy carbon electrode (GCE), the modified electrode showed a remarkable increase in the efficiency of the electrocatalytic oxidation of glucose, starting at around +0.70 V (vs. Ag/AgCl). The prepared sensor exhibited a high sensitivity of 784.14 µAmM⁻¹ cm⁻², a low detection limit of 2.1×10⁻⁷ M and a wide linear range from 2.5 µM to 4 mM (R=0.9997). More importantly, the LTA modified electrode was also relatively insensitive to commonly interfering species such as ascorbic acid (AA), uric acid (UA), dopamine (DA) in high potential. Moreover, the nonenzymatic sensor was applied to the determination of glucose in human serum samples and the results were in good agreement with clinical data. Electrodes modified with perovskite nanomaterials are highly promising for nonenzymatic electrochemical detection of glucose because of their high sensitivity, fast response, excellent stability and good reproducibility.
本文提出了一种基于钙钛矿 LaTiO3-Ag0.2（LTA）修饰电极的非酶葡萄糖生物传感器。通过扫描电子显微镜（SEM）和 X 射线衍射（XRD）分别对钙钛矿 LaTiO₃-Ag0.2 纳米材料的形貌和组成进行了表征。利用循环伏安法（CV）和电化学阻抗谱（EIS）对 LaTiO₃-Ag0.2（LTA）复合材料进行了电化学表征研究。在最佳条件下，CV 和计时电流法（I-t）研究表明，与裸玻碳电极（GCE）相比，修饰电极对葡萄糖的电催化氧化效率显著提高，起始电位约为+0.70 V（相对于 Ag/AgCl）。制备的传感器表现出高灵敏度 784.14 µAmM⁻¹ cm⁻²，低检测限 2.1×10⁻⁷ M 和从 2.5 µM 到 4 mM 的宽线性范围（R=0.9997）。更重要的是，LTA 修饰电极对高电位下常见干扰物质如抗坏血酸（AA）、尿酸（UA）、多巴胺（DA）也相对不敏感。此外，该无酶传感器还用于测定人血清样品中的葡萄糖，结果与临床数据吻合良好。由于具有高灵敏度、快速响应、优异的稳定性和良好的重现性，钙钛矿纳米材料修饰电极在葡萄糖的非酶电化学检测中具有广阔的应用前景。
