School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia.
Chemphyschem. 2013 Jul 22;14(10):2183-9. doi: 10.1002/cphc.201300040. Epub 2013 May 6.
Anti-fouling surfaces are of great importance for reducing background interference in biosensor signals. Oligo(ethylene glycol) (OEG) moieties are commonly used to confer protein resistance on gold, silicon and carbon surfaces. Herein, we report the modification of surfaces using electrochemical deposition of OEG aryl diazonium salts. Using electrochemical and contact angle measurements, the ligand packing density is found to be loose, which supports the findings of the fluorescent protein labelling that aryl diazonium OEGs confer resistance to nonspecific adsorption of proteins albeit lower than alkane thiol-terminated OEGs. In addition to protein resistance, aryl diazonium attachment chemistry results in stable modification. In common with OEG species on gold electrodes, OEGs with distal hydroxyl moieties do confer superior protein resistance to those with a distal methoxy group. This is especially the case for longer derivatives where superior coiling of the OEG chains is possible.
抗污表面对于降低生物传感器信号中的背景干扰非常重要。聚（乙二醇）（OEG）部分通常用于赋予金、硅和碳表面抗蛋白质的性质。在此，我们报告了使用电化学沉积 OEG 芳基重氮盐来修饰表面。通过电化学和接触角测量，发现配体堆积密度较松散，这支持了荧光蛋白标记的发现，即芳基重氮 OEG 赋予蛋白质非特异性吸附的抗性，尽管低于烷硫醇末端的 OEG。除了抗蛋白质吸附性，芳基重氮键合化学还导致稳定的修饰。与金电极上的 OEG 一样，带有远端羟基的 OEG 比带有远端甲氧基的 OEG 具有更好的蛋白质抗性。对于更长的衍生物尤其如此，因为 OEG 链可以更好地卷曲。
