蛋白质与聚乙二醇（OEG）自组装单分子层的相互作用：OEG稳定性、表面堆积密度和蛋白质吸附

Protein interactions with oligo(ethylene glycol) (OEG) self-assembled monolayers: OEG stability, surface packing density and protein adsorption.

作者信息

Li Lingyan, Chen Shengfu, Jiang Shaoyi

机构信息

Department of Chemical Engineering, University of Washington, Seattle, WA 98105, USA.

出版信息

J Biomater Sci Polym Ed. 2007;18(11):1415-27. doi: 10.1163/156856207782246795.

DOI:10.1163/156856207782246795

PMID:17961324

Abstract

We present a study of protein adsorption on oligo(ethylene glycol) (OEG) self-assembled monolayers (SAMs) at a range of OEG surface densities. OEG SAMs were formed in mixed ethanol and water solutions at different assembly temperatures to adjust the packing density of EG4-SAMs. These SAMs were characterized using X-ray photoelectron spectroscopy (XPS). Fibrinogen adsorption on these surfaces was measured by a surface plasmon resonance (SPR) sensor at different temperatures. This work is aimed at addressing three important issues for protein-OEG interactions, i.e., (i) OEG stability, (ii) the correlation between OEG surface densities and surface non-fouling properties, and (iii) protein adsorption on OEG surfaces at different temperatures.

摘要

我们展示了一项关于在一系列乙二醇（OEG）表面密度下蛋白质在乙二醇自组装单分子层（SAMs）上吸附的研究。在不同的组装温度下，于乙醇和水溶液的混合体系中形成OEG SAMs，以调节EG4 - SAMs的堆积密度。使用X射线光电子能谱（XPS）对这些SAMs进行表征。通过表面等离子体共振（SPR）传感器在不同温度下测量纤维蛋白原在这些表面上的吸附情况。这项工作旨在解决蛋白质与OEG相互作用的三个重要问题，即：（i）OEG的稳定性；（ii）OEG表面密度与表面抗污性能之间的相关性；以及（iii）不同温度下蛋白质在OEG表面的吸附情况。