Trueba M, Rodriguez P, Vallejo A I, Marino A, Sancho M J, Macarulla J M
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao/Spain.
Exp Clin Endocrinol. 1990 Apr;95(2):169-80. doi: 10.1055/s-0029-1210949.
Specific binding for progesterone has been determined in rat hepatocytes and mouse liver purified plasma membranes. The binding is saturable, reversible and temperature dependent. Two types of binding sites have been characterized in hepatocytes. The first is of high affinity and low binding capacity and the other one is of low affinity and high capacity of binding. In plasma membranes one type of specific binding site only exists. These high affinity sites are different from nuclear progesterone receptor, nuclear glucocorticoid receptor, digitalis receptor of Na+, K(+)-ATPase, transcortine and from corticoid binding sites determined previously in plasma membrane. We also have observed that specific progesterone binding to hepatocytes and plasma membrane is independent from the alpha and beta adrenergic receptors and from P-site adenosine receptor.
已在大鼠肝细胞和小鼠肝脏纯化质膜中测定了孕酮的特异性结合。这种结合是可饱和的、可逆的且依赖温度。在肝细胞中已鉴定出两种结合位点。第一种具有高亲和力和低结合容量，另一种具有低亲和力和高结合容量。在质膜中仅存在一种特异性结合位点。这些高亲和力位点不同于核孕酮受体、核糖皮质激素受体、Na +，K(+)-ATP酶的洋地黄受体、皮质激素转运蛋白以及先前在质膜中测定的皮质类固醇结合位点。我们还观察到，孕酮与肝细胞和质膜的特异性结合独立于α和β肾上腺素能受体以及P位点腺苷受体。
