Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics), University of the Balearic Islands (UIB) and CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Palma de Mallorca, Spain.
Mol Nutr Food Res. 2013 Aug;57(8):1456-67. doi: 10.1002/mnfr.201300126. Epub 2013 May 5.
We aimed to study the effects of free fatty acids (FFAs) alone and combined with the exercise mimetics adrenaline and 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) in the production of IL6, IL15 and Irisin in muscle cells, using a time-sequential model.
Differentiated C2C12 myotubes were treated with FFA, adrenaline or AICAR alone for 0, 1, 3, 8, 12 and 24 h and with double or triple combinations for 0, 3 and 24 h. Levels of mRNA in cells and protein in the medium were measured. Adrenaline, AICAR and FFA showed no significant effects on Irisin expression, while the presence in the culture of adrenaline and/or AICAR decreased IL15 mRNA expression. On contrary, the three signals showed a deep, rapid impact on the IL6 induction, especially when both AICAR and FFA were present.
The different response in IL6 versus IL15 regulation may be explained by their different energy-activating versus muscle-cell-hypertrophy suggested roles, considering that adrenaline and AMPK are involved in the activation of energy-generating pathways. Moreover, the results suggest FFAs are components that may regulate IL6 production and may have a role in muscle-adipose tissue crosstalk.
我们旨在使用时间序列模型研究游离脂肪酸（FFAs）单独作用以及与运动模拟物肾上腺素和 5-氨基咪唑-4-甲酰胺核糖核苷（AICAR）联合作用对肌肉细胞中 IL6、IL15 和鸢尾素产生的影响。
分化的 C2C12 肌管分别用 FFA、肾上腺素或 AICAR 单独处理 0、1、3、8、12 和 24 小时，并用双或三组合处理 0、3 和 24 小时。测量细胞内的 mRNA 水平和培养基中的蛋白质水平。肾上腺素、AICAR 和 FFA 对鸢尾素表达没有显著影响，而在培养物中存在肾上腺素和/或 AICAR 会降低 IL15 mRNA 的表达。相反，这三种信号对 IL6 的诱导有深刻、快速的影响，尤其是当 AICAR 和 FFA 同时存在时。
IL6 与 IL15 调节的不同反应可能可以用它们不同的能量激活与肌肉细胞肥大的作用来解释，因为肾上腺素和 AMPK 参与了能量产生途径的激活。此外，结果表明 FFA 可能是调节 IL6 产生的成分，并可能在肌肉-脂肪组织相互作用中发挥作用。
