欧芹质体蓝素的微观异质性

Microheterogeneity of parsley plastocyanin.

作者信息

Dimitrov M I, Donchev A A, Egorov T A

机构信息

Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.

出版信息

FEBS Lett. 1990 Jun 4;265(1-2):141-5. doi: 10.1016/0014-5793(90)80904-w.

DOI:10.1016/0014-5793(90)80904-w

PMID:2365050

Abstract

A procedure for isolation of two iso-plastocyanins from parsley has been described here. Three consecutive chromatographic steps on DE-52-Whatman cellulose were applied for isolation of two total plastocyanin (PC) fractions, oxidized [PC(II)] and reduced [PC(I)]. By chromatofocusing of PC(II) on Polybuffer exchanger 74 two different plastocyanins, designated as plastocyanin a (PCa) and plastocyanin b (PCb), were obtained. The isoelectric points (pI) of PCa and PCb at 10 degrees C are 4.16 and 4.14, respectively. The complete amino acid sequences of PCa and PCb were determined. The two iso-proteins consist of 97 amino acid residues and differ only at sequence position 53, where Glu in PCa is replaced by Asp in PCb.

摘要

本文描述了从欧芹中分离两种同工质体蓝素的方法。在DE-52-沃特曼纤维素上进行了三个连续的色谱步骤，以分离出两种总质体蓝素（PC）组分，即氧化型[PC(II)]和还原型[PC(I)]。通过在Polybuffer交换剂74上对PC(II)进行色谱聚焦，获得了两种不同的质体蓝素，分别命名为质体蓝素a（PCa）和质体蓝素b（PCb）。在10℃时，PCa和PCb的等电点（pI）分别为4.16和4.14。测定了PCa和PCb的完整氨基酸序列。这两种同工蛋白由97个氨基酸残基组成，仅在序列位置53处有所不同，其中PCa中的谷氨酸被PCb中的天冬氨酸取代。