一种从豌豆（Pisum sativum）叶片中快速纯化质体蓝素的新方法。
A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves.
作者信息
Ashton A R, Anderson L E
出版信息
Biochem J. 1981 Jan 1;193(1):375-8. doi: 10.1042/bj1930375.
Abstract
Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.
摘要
质体蓝素在高浓度（大于3M）的硫酸铵中可溶，但在这些条件下会紧密吸附到未取代的琼脂糖珠上。利用这一观察结果，通过两步色谱法从豌豆（Pisum sativum）中纯化质体蓝素。用低离子强度缓冲液洗脱结合在琼脂糖上的质体蓝素，随后通过DEAE-纤维素色谱法将其纯化至同质。
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