青藤碱在变应性鼻炎小鼠模型治疗中的作用及其机制

[The role of sinomenine in treatment of allergic rhinitis mice model and its mechanism].

作者信息

Chen Zhe, Tao Zezhang, Zhang Nana, Ren Jie, Deng Yuqin, Xiao Bakui

机构信息

Department of Otolaryngology-Head and Neck Surgery, People's Hospital of Wuhan University, Wuhan, 430060, China.

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2013 Jan;27(2):81-4.

PMID:23650707

Abstract

OBJECTIVE

To explore the role of sinomenine in treatment of allergic rhinitis mice model and its possible mechanism.

METHOD

We used ovalbumin (OVA) to make allergic rhinitis model of BALB/c mice. Saline was used in the control group. When we challenged the mice with OVA intranasally, the mice in sinomenine treatment group were feed by the food containing sinomenine. Mice were then killed 24 h after the last OVA challenge. The noses of mice from each group were removed en bloc and fixed, then each section was stained with hematoxylin and eosin. ELISA assay was used to measure the concentration of anti-OVA IgE, IL-4 and IFN-gamma. The proteins expressive level of T-bet and GATA3 were examined.

RESULT

Nasal mucosa of the mice in sinomenine treatment group were not hyperplasia and without obvious infiltration of eosinophils. The concentration of anti-OVA IgE, IL-4 and IFN-gamma in the serum and T-bet and GATA3 expression levels of sinomenine treatment group were lower than those of allergic rhinitis group.

CONCLUSION

The sinomenine can be used to treat allergic rhinitis mice, and the mechanism may rely on the improvements of the Th1/Th2 imbalance.

摘要

目的

探讨青藤碱对变应性鼻炎小鼠模型的治疗作用及其可能机制。

方法

采用卵清蛋白（OVA）制备BALB/c小鼠变应性鼻炎模型，对照组给予生理盐水。当用OVA对小鼠进行鼻内激发时，青藤碱治疗组小鼠给予含青藤碱的食物。在最后一次OVA激发后24小时处死小鼠。将每组小鼠的鼻子整块取下并固定，然后对每个切片进行苏木精和伊红染色。采用ELISA法检测抗OVA IgE、IL-4和IFN-γ的浓度。检测T-bet和GATA3的蛋白表达水平。