Ryzhakova O S, Zavalishina L É, Andreeva Iu Iu, Solov'eva N I
Biomed Khim. 2013 Jan-Feb;59(1):55-64. doi: 10.18097/pbmc20135901055.
Interstitial collagenase and gelatinases are matrix metalloproteinases (MMP), which play the key role in tumor invasion and metastasis. The aim of this study was to elucidate the peculiarities of expression of interstitial collagenase (MMP-1), gelatinases A and B (MMP-2 and MMP-9) and their endogenous tissue inhibitors TIMP-1 and TIMP-2 as invasive factors of squamous cell carcinomas (SCC) of human cervical cancer. The study was carried out using 24 specimens of SCC and 11 specimens of adjacent to tumor morphologically normal tissue. All carcinoma specimens expressed E7 HPV-16 gene. It was shown that the increase of MMP-1 and MMP-9 expression and low of TIMP-1 and TIMP-2 expression makes the main contribution to the destructive (invasive) potential of SCC. The change of MMP-2 expression is not so significant and it is less influenced to the destructive potential. Moreover, substantial expression of MMP-1, MMP-2 and MMP-9 was registered in the specimens of morphologically normal adjoining to tumor tissue. This expression was found to make an additional contribution to the destructive potential of cervical tumor.
间质胶原酶和明胶酶属于基质金属蛋白酶（MMP），在肿瘤侵袭和转移过程中起关键作用。本研究旨在阐明间质胶原酶（MMP-1）、明胶酶A和B（MMP-2和MMP-9）及其内源性组织抑制剂TIMP-1和TIMP-2作为人宫颈癌鳞状细胞癌（SCC）侵袭因子的表达特点。研究采用了24份SCC标本和11份肿瘤旁形态学正常组织标本。所有癌组织标本均表达E7 HPV-16基因。结果表明，MMP-1和MMP-9表达增加以及TIMP-1和TIMP-2表达降低对SCC的破坏（侵袭）潜能起主要作用。MMP-2表达的变化不显著，对破坏潜能的影响较小。此外，在肿瘤旁形态学正常组织标本中也检测到MMP-1、MMP-2和MMP-9的大量表达。发现这种表达对宫颈肿瘤的破坏潜能有额外贡献。
