Rahimi Zohreh, Ahmadi Reza, Vaisi-Raygani Asad, Rahimi Ziba, Bahrehmand Fariborz, Parsian Abbas
Medical Biology Research Center .
J Matern Fetal Neonatal Med. 2013 Nov;26(16):1590-4. doi: 10.3109/14767058.2013.795534. Epub 2013 May 23.
To determine the butyrylcholinesterase (BChE) activity and phenotypes in preeclampsia and its possible association with lipid and lipoprotein metabolism and oxidative stress in preeclamptic women.
In a case-control study, 101 pregnant women with normal pregnancy and 198 women with preeclampsia from Western Iran were studied. The serum BChE activity and phenotypes were measured using spectrophotometric method. The apolipoprotein E (APOE) genotypes were identified using PCR-RFLP. The serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined by HPLC and commercial kits, respectively.
The BChE activity and the frequency of non-usual BChE phenotype in preeclamptic women were significantly lower and higher, respectively compared to controls. There was a higher BChE activity in the presence of APOE ε3ε4 compared to ε3ε3 genotype in preeclamptic women. In addition, there were significant positive correlations between BChE activity and the levels of low-density lipoprotein-cholesterol, high-density lipoprotein-cholesterol, total cholesterol (TC) and TAC. However, there was a negative but significant correlation between BChE activity and MDA level.
Our study for the first time indicated that BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress.
确定子痫前期患者的丁酰胆碱酯酶（BChE）活性和表型，及其与子痫前期患者脂质和脂蛋白代谢以及氧化应激的可能关联。
在一项病例对照研究中，对来自伊朗西部的101例正常妊娠孕妇和198例子痫前期患者进行了研究。采用分光光度法测定血清BChE活性和表型。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）鉴定载脂蛋白E（APOE）基因型。分别通过高效液相色谱法（HPLC）和商业试剂盒测定血清丙二醛（MDA）水平和总抗氧化能力（TAC）。
与对照组相比，子痫前期患者的BChE活性显著降低，而非典型BChE表型的频率显著升高。子痫前期患者中，与ε3ε3基因型相比，存在APOE ε3ε4时BChE活性更高。此外，BChE活性与低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、总胆固醇（TC）水平和TAC之间存在显著正相关。然而，BChE活性与MDA水平之间存在负相关但显著相关。
我们的研究首次表明，BChE活性可能通过影响脂质和脂蛋白代谢以及氧化应激参与子痫前期的发病机制。
