Exp Dermatol. 2013 Jun;22(6):423-5. doi: 10.1111/exd.12149. Epub 2013 May 8.
Development of epidermis creates stratified epithelium with different sets of ion-transporting enzymes in its layers. We have characterized expression of Na,K- and H,K-ATPase α and β subunits and FXYD isoforms in rat skin. Maturation of rat skin from newborn to adult is associated with an increase in FXYD4 and a decrease of Na,K-ATPase α1-isoform, ATP1B4 and FXYD6 transcripts. Na,K-ATPase of rat epidermis is represented predominantly by α1 and β3 isoforms. Keratinization is associated with the loss of the Na,K-ATPase α-subunit and an enrichment of αng. Na,K-ATPase α1 is abundant in the innermost layer, stratum basale, where it is lacking in basal membranes, thus indicating lateroapical polarization of Na,K-ATPase. Immunocytochemical detection of Na,K-ATPase in Xenopus laevis skin shows that cellular and subcellular localization of the enzyme has a pattern highly similar to that of mammals: basolateral in glandular epithelium and lateroapical in epidermis.
表皮的发育使不同层次的具有不同离子转运酶的复层上皮形成。我们已经描述了 Na，K-和 H，K-ATPase α和β亚基以及 FXYD 同工型在大鼠皮肤中的表达。从新生到成年大鼠皮肤的成熟与 FXYD4 的增加和 Na，K-ATPase α1-同工型、ATP1B4 和 FXYD6 转录物的减少有关。大鼠表皮的 Na，K-ATPase 主要由 α1 和 β3 同工型组成。角化与 Na，K-ATPase α 亚基的丧失和 αng 的富集有关。Na，K-ATPase α1 在最内层，基底层中含量丰富，而在基底膜中缺乏，这表明 Na，K-ATPase 的侧向顶端极化。用 Xenopus laevis 皮肤进行的 Na，K-ATPase 免疫细胞化学检测表明，该酶的细胞和亚细胞定位与哺乳动物非常相似：在腺上皮中是基底外侧，在表皮中是侧向顶端。
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