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在蚕（Bombyx mori）中表达的鲨鱼肝基因衍生的活性肽：口服重组蛋白的初步研究。

A shark liver gene-derived active peptide expressed in the silkworm, Bombyx mori: preliminary studies for oral administration of the recombinant protein.

机构信息

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.

出版信息

Mar Drugs. 2013 May 7;11(5):1492-505. doi: 10.3390/md11051492.

DOI:10.3390/md11051492
PMID:23652883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3707157/
Abstract

Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future.

摘要

鲨鱼肝活性肽（APSL）是一种来自尖齿鲨的细胞因子，可刺激肝脏再生并保护胰腺。为了研究口服重组 APSL（rAPSL）对 2 型糖尿病动物模型的影响，克隆了 APSL 基因，并利用家蚕杆状病毒表达载体系统（BEVS）在家蚕 N 细胞（BmN 细胞）、蚕幼虫和蚕蛹中表达 APSL。结果表明，rAPSL 可显著降低链脲佐菌素诱导的 2 型糖尿病小鼠的血糖水平。对小鼠胰腺组织石蜡切片的分析表明，rAPSL 可有效保护小鼠胰岛免受链脲佐菌素诱导的损伤。与正常蚕蛹粉相比，表达 rAPSL 的蛹粉表现出更大的保护作用，这些结果表明 rAPSL 具有作为未来治疗糖尿病的口服药物的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/1d72dc23c1ca/marinedrugs-11-01492-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/8ba48ab000b2/marinedrugs-11-01492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/f7275a77d0c9/marinedrugs-11-01492-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/24ae22efc234/marinedrugs-11-01492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/c5234dbebe80/marinedrugs-11-01492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/f2ef4097dc08/marinedrugs-11-01492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/8807ab09f2af/marinedrugs-11-01492-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/c4902a463ea1/marinedrugs-11-01492-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/1d72dc23c1ca/marinedrugs-11-01492-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/8ba48ab000b2/marinedrugs-11-01492-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/f7275a77d0c9/marinedrugs-11-01492-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/24ae22efc234/marinedrugs-11-01492-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/c5234dbebe80/marinedrugs-11-01492-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/f2ef4097dc08/marinedrugs-11-01492-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/8807ab09f2af/marinedrugs-11-01492-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/c4902a463ea1/marinedrugs-11-01492-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/427e/3707157/1d72dc23c1ca/marinedrugs-11-01492-g008.jpg

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