College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, Korea.
Molecules. 2013 May 7;18(5):5235-50. doi: 10.3390/molecules18055235.
Two bisbenzylisoquinoline alkaloids, two morphine alkaloids, one aporphine alkaloid, syringaresinol and aristolochic acid І were selected as marker compounds and simultaneously analyzed using an ultra-high pressure liquid chromatography-diode array detection (UHPLC-DAD) method. These marker compounds were used for the quality control of Fangchi species of different origins, including Sinomenium acutum, Stephania tetrandra, Cocculus trilobus and Aristolochia fangchi. A reversed-phase UHPLC-DAD method was developed and validated for the simultaneous quantification of structurally diverse markers in different Fangchi species. In addition, an UHPLC-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method was used for marker identification in Fangchi species, which provided diagnostic MS/MS spectral patterns that were dependent upon the marker structures. The UHPLC-MS/MS data were used to confirm and complement the UHPLC-DAD quality evaluation results. Additionally, magnoflorine and syringaresinol were observed for the first time in S. tetrandra and C. trilobus, respectively. Twenty different Fangchi species samples were analyzed for aristolochic acid I, syringaresinol and the alkaloids using the UHPLC-DAD and MS/MS method. Based on the levels of markers and principal component analysis (PCA), this method allowed for the clear classification of the samples into four different groups representing samples originating from the four species.
两种双苄基异喹啉生物碱、两种吗啡生物碱、一种阿朴啡生物碱、丁香脂素和马兜铃酸Ⅰ被选为标记化合物，并使用超高效液相色谱-二极管阵列检测（UHPLC-DAD）方法同时进行分析。这些标记化合物被用于不同来源的防己属物种的质量控制，包括青风藤、粉防己、三角叶马兜铃和广防己。建立并验证了一种反相 UHPLC-DAD 方法，用于同时定量不同防己属物种中结构多样的标记化合物。此外，还使用 UHPLC-电喷雾电离串联质谱（ESI-MS/MS）方法对防己属物种中的标记化合物进行鉴定，提供了依赖于标记结构的诊断性 MS/MS 光谱模式。UHPLC-MS/MS 数据用于确认和补充 UHPLC-DAD 质量评估结果。此外，在粉防己和三角叶马兜铃中首次观察到了厚朴碱和丁香脂素。使用 UHPLC-DAD 和 MS/MS 方法分析了 20 种不同的防己属物种样品中的马兜铃酸Ⅰ、丁香脂素和生物碱。基于标记物和主成分分析（PCA）的水平，该方法能够清楚地将样品分为代表来自四个物种的四个不同组的样品。
