一种用于测定结合蛋白A的单克隆抗体的微量酶联免疫吸附测定法。
A microELISA useful for determination of protein A-binding monoclonal antibodies.
作者信息
Larsson A, Holmdahl R
机构信息
Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.
出版信息
Hybridoma. 1990 Jun;9(3):289-94. doi: 10.1089/hyb.1990.9.289.
Affinity chromatography on protein A columns is widely used for the purification of monoclonal antibodies. However, some monoclonal antibodies do not bind protein A. We have developed a sandwich-ELISA for determination of the protein A binding capacity of antibodies. This utilizes microtitre plates coated with protein A, and labeled chicken antibodies for detection of bound antibody. Chicken IgG is one of the few antibodies that does not react with protein A, and can thus be used without the problem of a high background.
蛋白A柱亲和层析被广泛用于单克隆抗体的纯化。然而，一些单克隆抗体不与蛋白A结合。我们开发了一种夹心酶联免疫吸附测定法来测定抗体的蛋白A结合能力。该方法利用包被有蛋白A的微量滴定板，以及用于检测结合抗体的标记鸡抗体。鸡免疫球蛋白G是少数不与蛋白A反应的抗体之一，因此可以使用而不会出现高背景的问题。
Zotero 插件