通过聚乙二醇沉淀法对双链DNA进行大小分级分离。
Size fractionation of double-stranded DNA by precipitation with polyethylene glycol.
作者信息
Lis J T, Schleif R
出版信息
Nucleic Acids Res. 1975 Mar;2(3):383-9. doi: 10.1093/nar/2.3.383.
We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG+.. Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 times 10-7 to 1 times 10-5 daltons. The effects on PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies show PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.
我们发现，不同分子量的DNA分子可通过用聚乙二醇（PEG）选择性沉淀来分离。较高分子量的DNA在比低分子量DNA更低的PEG浓度下沉淀。双链DNA至少在3×10⁻⁷至1×10⁻⁵道尔顿的范围内可以分级分离。已确定了PEG浓度、氯化钠浓度、DNA浓度、pH值、二价离子、沉淀时间和离心力的影响。这些研究表明，PEG沉淀为DNA提供了一种尺寸分级方法，该方法方便、容量大且适用于广泛的条件。然而，分辨率不高，只有当两个物种的分子量相差至少两倍时，它们的分离接近100%。
Zotero 插件