核糖核酸酶T1与DNA的相互作用。
The interaction of ribonuclease T 1 with DNA.
作者信息
Walz F G, Biddlecome S, Hooverman L
出版信息
Nucleic Acids Res. 1975 Jan;2(1):11-20. doi: 10.1093/nar/2.1.11.
The interaction of RNase T1 with calf thymus DNA was studied using uv difference spectroscopy and the effect of the enzyme on DNA melting. There was no indication of RNase T1 binding with native DNA. A prominent difference spectrum for RNase T1 binding with denatured DNA (d-DNA) was observed at pH 5, 25 degrees and low ionic strength (mu = .01 M) which was depressed at higher ionic strength and pH. The normalized difference spectrum at mu = .01 M, pH 5 and 25 degrees can be interpreted as indicating an interaction of an exposed guanine residue directly with the enzyme and a coupling of this process with the "melting" of short folded segments of d-DNA. The apparent association constant calculated per M guanine residues was 2.4 X 10-4 M-1 under these conditions. The results are discussed in reference to comparable studies on the interaction of RNase T1 with RNA and small guanine ligands.
利用紫外差光谱法以及该酶对DNA解链的影响，对核糖核酸酶T1与小牛胸腺DNA的相互作用进行了研究。没有迹象表明核糖核酸酶T1与天然DNA结合。在pH 5、25摄氏度和低离子强度（μ = 0.01 M）条件下，观察到核糖核酸酶T1与变性DNA（d-DNA）结合时出现明显的差光谱，在较高离子强度和pH条件下该差光谱减弱。在μ = 0.01 M、pH 5和25摄氏度时的归一化差光谱可解释为表明一个暴露的鸟嘌呤残基直接与该酶相互作用，并且此过程与d-DNA短折叠片段的“解链”相偶联。在这些条件下，每摩尔鸟嘌呤残基计算得到的表观缔合常数为2.4×10⁻⁴ M⁻¹。结合对核糖核酸酶T1与RNA以及小的鸟嘌呤配体相互作用的可比研究，对这些结果进行了讨论。
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