Tschumper R C, Johnson D H
Mayo Clinic, Rochester, Minnesota 55905.
Invest Ophthalmol Vis Sci. 1990 Jul;31(7):1327-31.
The difference in trabecular meshwork cellularity between fellow eyes was determined in a series of seven pairs of normal human eyes. Variations in cellularity between different quadrants of individual eyes also were determined. Characterization of these differences and development of appropriate sampling techniques to determine trabecular cellularity has become important in light of recent studies that find a decrease in trabecular cell numbers with age and with primary open-angle glaucoma, and the apparent stimulation of trabecular cell division by laser trabeculoplasty. The difference in absolute cell numbers (nuclei per section) between fellow eyes was 12.8% +/- 4.3% (standard error of the mean, SEM). The difference in cellularity (nuclei per solid tissue area) was 9.4% +/- 4.5% (SEM) in a subgroup of five pairs of these eyes. The coefficient of variation between different quadrants of an eye was 18.4% for absolute cell numbers and 17.3% for cellularity for the entire group of eyes. Sample size calculations indicate that for future studies, four pairs of eyes would be needed to detect a 20% difference in absolute cell numbers between fellow eyes and three pairs needed for detecting a similar difference in cellularity (alpha = 0.05, beta = 0.1). At least two quadrants per eye should be examined.
在一系列七对正常人类眼睛中，确定了双眼小梁网细胞密度的差异。还确定了单眼不同象限之间细胞密度的变化。鉴于最近的研究发现小梁细胞数量随年龄增长和原发性开角型青光眼而减少，以及激光小梁成形术对小梁细胞分裂有明显刺激作用，表征这些差异并开发合适的采样技术以确定小梁细胞密度变得很重要。双眼之间绝对细胞数量（每切片细胞核数）的差异为12.8%±4.3%（均值标准误差，SEM）。在这七对眼睛中的五对眼睛组成的亚组中，细胞密度（每实体组织面积细胞核数）的差异为9.4%±4.5%（SEM）。对于整个眼睛组，一只眼睛不同象限之间的变异系数，绝对细胞数量为18.4%，细胞密度为17.3%。样本量计算表明，对于未来的研究，需要四对眼睛来检测双眼之间绝对细胞数量20%的差异，需要三对眼睛来检测细胞密度的类似差异（α = 0.05，β = 0.1）。每只眼睛至少应检查两个象限。
