Slaska Brygida, Grzybowska-Szatkowska Ludmila, Surdyka Magdalena, Nisztuk Sylwia, Rozanska Dorota, Rozanski Pawel, Smiech Anna, Orzelski Maciej
Department of Biological Bases of Animal Production, University of Life Sciences in Lublin , Lublin , Poland .
Mitochondrial DNA. 2014 Jun;25(3):238-43. doi: 10.3109/19401736.2013.792054. Epub 2013 May 8.
Abstract The aim of the conducted investigations was to identify differences in the D-loop nucleotide sequence between neoplastic tissue, normal tissue, and blood and to determine their correlation with the type of cancer in dogs. In 62.5% of the analyzed tumors of epithelial origin and 25% tumors of mesenchymal origin, substitution was detected within the D-loop sequence between the neoplastic tissue, normal tissue, and blood. Two mutations occurring in the carcinogenic process in position T15620C have been identified in epithelioma glandulae sebacei and carcinoma planoepithelialae keratodes. Blood and cancer heteroplasmy was diagnosed for carcinoma planoepithelialae keratodes and "Comedo" carcinoma. The results of the study indicate that polymorphic changes in the D-loop sequence promote carcinogenesis in dogs. Heteroplasmy diagnosed in blood and tumor cells and absence thereof in normal tissue may imply mtDNA recombination. High prevalence of mtDNA mutations in canine tumors may suggest mtDNA genetic instability, which is likely to play a role in carcinogenesis.
摘要 所开展调查的目的是确定肿瘤组织、正常组织和血液之间D环核苷酸序列的差异，并确定它们与犬类癌症类型的相关性。在62.5%的分析上皮起源肿瘤和25%的间充质起源肿瘤中，检测到肿瘤组织、正常组织和血液之间的D环序列存在替代。在皮脂腺上皮瘤和角化扁平上皮癌中已鉴定出在致癌过程中发生在T15620C位置的两个突变。对角化扁平上皮癌和“粉刺样”癌诊断出血液和癌症异质性。研究结果表明，D环序列中的多态性变化促进犬类致癌作用。在血液和肿瘤细胞中诊断出异质性而在正常组织中不存在异质性可能意味着线粒体DNA重组。犬类肿瘤中线粒体DNA突变的高发生率可能表明线粒体DNA遗传不稳定性，这可能在致癌作用中发挥作用。
