离体灌注大鼠肝脏合成血清抗胰蛋白酶

Serum antitrypsin synthesis by the isolated perfused rat liver.

作者信息

Rowley P T, Miller L L

出版信息

Proc Soc Exp Biol Med. 1975 Jan;148(1):145-50. doi: 10.3181/00379727-148-38493.

Abstract

The isolated perfused rat liver synthesizes antitrypsin activity in a linear fashion as long as 24 hr. The rate of synthesis may be ten times the rate necessary for physiologic levels in vivo. These data are compatible with the liver as the principal site of synthesis of serum antitryptic activity in the rat. The antitrypsin activity of the rat perfusate resembles that of normal human plasma in liability to heat (56 degrees) and to acid (below pH 6) and in apparent molecular size (elution from Sephadex G-200).

摘要

离体灌注的大鼠肝脏能够以线性方式合成抗胰蛋白酶活性，长达24小时。合成速率可能是体内生理水平所需速率的十倍。这些数据表明，肝脏是大鼠血清抗胰蛋白酶活性的主要合成部位。大鼠灌注液中的抗胰蛋白酶活性在对热（56摄氏度）和酸（pH值低于6）的敏感性以及表观分子大小（从葡聚糖G-200洗脱）方面与正常人血浆相似。