Maragoudakis M E, Kalinsky H J, Lennane J
Proc Soc Exp Biol Med. 1975 Mar;148(3):606-10. doi: 10.3181/00379727-148-38593.
Octanoate is avidly incorporated into triglycerides by isolated rat adipocytes in the presence of glucose via direct esterification without prior beta-oxidation to acetyl CoA. This was shown by separation of the products formed from (1-14C) octanoate into lipid classes using Florisil columns, and after alkaline hydrolysis of the triglyceride fraction, by cochromatogrpahy with authentic fatty acids on reverse-phase Celite columns. The relative contribution of (U-14C) glucose and (1-14C) octanoate to triglyceride synthesis and CO2 formation were studied under a variety of conditions. Concentrations of octanoate below 0.5 mM have a stimulatory effect on the conversion of (U-14C) glucose to CO2, triglycerides and esterified fatty acids. However, a marked depression of fatty acid synthesis from (U-14C) glucose was observed in the presence of millimolar concentrations of octanoate. Octanoate had no effect on the esterification of palmitate, but palmitate strongly depressed the ability of rat adipocytes to esterify octanoate.
在有葡萄糖存在的情况下，离体大鼠脂肪细胞可通过直接酯化作用将辛酸迅速掺入甘油三酯中，而无需先将其β氧化成乙酰辅酶A。这是通过使用弗罗里硅土柱将由（1-¹⁴C）辛酸形成的产物分离成脂质类别来证明的，并且在甘油三酯部分进行碱性水解后，通过在反相硅藻土柱上与纯脂肪酸进行共色谱分析来证明的。在各种条件下研究了（U-¹⁴C）葡萄糖和（1-¹⁴C）辛酸对甘油三酯合成和二氧化碳形成的相对贡献。低于0.5 mM的辛酸浓度对（U-¹⁴C）葡萄糖转化为二氧化碳、甘油三酯和酯化脂肪酸具有刺激作用。然而，在存在毫摩尔浓度的辛酸时，观察到（U-¹⁴C）葡萄糖的脂肪酸合成明显受到抑制。辛酸对棕榈酸的酯化没有影响，但棕榈酸强烈抑制大鼠脂肪细胞酯化辛酸的能力。
