Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Anal Bioanal Chem. 2013 Jul;405(17):5679-85. doi: 10.1007/s00216-013-6983-1. Epub 2013 May 9.
In this review, we summarize recent advances in the development of molecular recognition components based on the biomolecular scaffolds of proteins and nucleic acids for specific recognition of miscellaneous targets. In addition to the widely adopted recombinant antibody fragments, designed ankyrin repeat proteins and modular peptide repeats of transcription-activator-like effectors for base-specific recognition of DNA sequence are also briefly introduced. For the nucleic acid based molecular recognition systems, aptamers, including slow off-rate modified aptamers, DNAzymes, and synthetic DNA-like oligomers for versatile biorecognition are described. Finally, we discuss the remaining challenges and future research directions in the field.
在这篇综述中，我们总结了基于蛋白质和核酸生物分子支架的分子识别组件在特定识别各种靶标方面的最新进展。除了广泛采用的重组抗体片段外，我们还简要介绍了用于 DNA 序列碱基特异性识别的设计锚蛋白重复蛋白和转录激活子样效应物的模块化肽重复。对于基于核酸的分子识别系统，我们描述了适体，包括慢解离修饰适体、DNA 酶和用于多功能生物识别的合成类似 DNA 的寡聚物。最后，我们讨论了该领域尚存的挑战和未来的研究方向。
