Central Unit for Ionbeams and Radionuclides (RUBION), Ruhr-University Bochum, Bochum, Germany.
Sci Rep. 2013;3:1806. doi: 10.1038/srep01806.
The migration of oligodendrocyte progenitor cells (OPCs) to the white matter is an indispensable requirement for an intact brain function. The mechanism of cell migration in general is not yet completely understood. Nevertheless, evidence is accumulating that besides the coordinated rearrangement of the cytoskeleton, a finetuned interplay of ion and water fluxes across the cell membrane is essential for cell migration. One part of a general hypothesis is that a local volume increase towards the direction of movement triggers a mechano-activated calcium influx that regulates various procedures at the rear end of a migrating cell. Here, we investigated cell volume changes of migrating OPCs using scanning ion conductance microscopy. We found that during accelerated migration OPCs undergo an increase in the frontal cell body volume. These findings are supplemented with time lapse calcium imaging data that hint an increase in calcium content the frontal part of the cell soma.
少突胶质前体细胞（OPC）向白质迁移是大脑功能完整所必需的。细胞迁移的机制尚未完全了解。然而，越来越多的证据表明，除了细胞骨架的协调重排外，细胞膜跨膜离子和水通量的精细相互作用对于细胞迁移也是必不可少的。一个普遍假设的一部分是，朝向运动方向的局部体积增加会触发机械激活的钙内流，从而调节迁移细胞后端的各种过程。在这里，我们使用扫描离子电导显微镜研究了迁移中的 OPC 的细胞体积变化。我们发现，在加速迁移过程中，OPC 的前体细胞体积增加。这些发现补充了延时钙成像数据，这些数据提示细胞体前部钙含量增加。
