Australian Center for Research on Separation Science, School of Pharmacy, Faculty of Health Sciences, University of Tasmania, Australia.
Electrophoresis. 2013 Jun;34(11):1465-82. doi: 10.1002/elps.201200646. Epub 2013 May 8.
Chemical characterization and monitoring of fermentation broths and cell culture media provide significant information on the changes occurring within these complex and dynamic systems. Analytical methods based on CE in capillaries and microchips are attractive for integration in instrumental tools to obtain this critical data, improving the understanding and control of bioprocesses. In this review, the use of CE for chemical characterization and monitoring fermentations is discussed, organized by analyte class, including organic acids, pharmaceuticals, proteins, sugars, amino acids, and metabolites published between 1992 and October 2012. A section is dedicated to the roles CE plays throughout the wine making process, where applications range from characterization and increase in fundamental understanding of the fermentation to forensic applications, verifying the authenticity of the wine.
化学特性分析和发酵液及细胞培养基的监测为了解这些复杂和动态系统中的变化提供了重要信息。基于毛细管和微芯片的 CE 分析方法非常适合集成到仪器工具中，以获得这些关键数据，从而改善对生物过程的理解和控制。在这篇综述中，根据分析物类别讨论了 CE 在发酵化学特性分析和监测中的应用，包括有机酸、药物、蛋白质、糖、氨基酸和代谢物，时间跨度为 1992 年至 2012 年 10 月。其中有一部分专门讨论了 CE 在葡萄酒酿造过程中的作用，其应用范围从发酵过程的基本特性和深入理解到法医应用，包括验证葡萄酒的真实性。
