Department of Genetics, Hebei Medical University, Hebei Key Lab of Laboratory Animal, Shijiazhuang, Hebei Province 050017, People's Republic of China.
Genome. 2013 Mar;56(3):147-54. doi: 10.1139/gen-2012-0178. Epub 2013 Mar 28.
DNA segmentation methods were used to study which fragments of the human IFNγ gene possess enhancer activity. The human IFNγ gene was divided into 240-bp fragments, which were inserted between the GFP gene and the Alu tandem sequence to determine whether the inserted sequences eliminate the inhibition induced by the Alu tandem sequence. We found that five different 240-bp fragments (FUIFN3F3R, IFN4F4R, IFN6F6R, IFN21F21R, and IFN22F22R) and two 60-bp core sequences (IFN6-2F2R and IFN21-3-4F3-4R) derived from the IFNγ gene contain enhancers that can activate the GFP reporter gene. These enhancers may be targets of IFNγ gene expression regulation.
DNA 片段化方法被用于研究人类 IFNγ 基因的哪些片段具有增强子活性。将人类 IFNγ 基因分成 240bp 的片段，插入 GFP 基因和 Alu 串联序列之间，以确定插入序列是否消除了 Alu 串联序列诱导的抑制作用。我们发现，来自 IFNγ 基因的五个不同的 240bp 片段（FUIFN3F3R、IFN4F4R、IFN6F6R、IFN21F21R 和 IFN22F22R）和两个 60bp 核心序列（IFN6-2F2R 和 IFN21-3-4F3-4R）含有能够激活 GFP 报告基因的增强子。这些增强子可能是 IFNγ 基因表达调控的靶点。
