Rebouças Emanuela L, Costa José J N, Passos Maria J, Silva Anderson W B, Rossi Rodrigo O D S, van den Hurk Robert, Silva José R V
Biotechnology Nucleus of Sobral - NUBIS,Federal University of Ceara,Sobral,CE,Brazil.
Department of Pathobiology,Faculty of Veterinary Medicine,Utrecht University,Utrecht,The Netherlands.
Zygote. 2014 Nov;22(4):521-32. doi: 10.1017/S0967199413000166. Epub 2013 May 9.
This study investigated mRNA levels for insulin-like growth factors (IGFs) IGF1 (IGF-I) and IGF2 (IGF-II), IGF receptors (IGF1R and IGF2R), and binding proteins (IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6) in bovine follicles of 0.2, 0.5 or 1.0 mm in diameter. mRNA expression levels in in vitro cultured follicles that reached approximately 0.5 mm were compared with that of in vivo grown follicles. IGF1R and IGF2R expression levels in 0.5 mm in vivo follicles were higher than in 1.0 or 0.2 mm follicles, respectively. IGFBP-1, IGFBP-2. IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6 showed variable expression in the follicular size classes analyzed. In vitro grown follicles had significantly reduced expression levels for IGF1, IGF1R, IGFBP-3, IGFBP-5 and IGFBP-6 mRNA when compared with 0.2 mm follicles, but, when compared with in vivo grown follicles (0.5 mm), only IGFBP-1, IGFBP-2, IGFBP-3 and IGFBP-6 showed a reduction in their expression. In conclusion, IGFs, their receptors and IGFBPs showed variable expression of mRNA levels in the follicular size classes analyzed.
本研究调查了直径为0.2、0.5或1.0毫米的牛卵泡中胰岛素样生长因子（IGFs）IGF1（IGF - I）和IGF2（IGF - II）、IGF受体（IGF1R和IGF2R）以及结合蛋白（IGFBP - 1、IGFBP - 2、IGFBP - 3、IGFBP - 4、IGFBP - 5和IGFBP - 6）的mRNA水平。将体外培养至约0.5毫米的卵泡中的mRNA表达水平与体内生长的卵泡进行比较。0.5毫米的体内卵泡中IGF1R和IGF2R的表达水平分别高于1.0毫米或0.2毫米的卵泡。IGFBP - 1、IGFBP - 2、IGFBP - 3、IGFBP - 4、IGFBP - 5和IGFBP - 6在所分析的卵泡大小类别中表现出不同的表达。与0.2毫米的卵泡相比，体外生长的卵泡中IGF1、IGF1R、IGFBP - 3、IGFBP - 5和IGFBP - 6 mRNA的表达水平显著降低，但与体内生长的卵泡（0.5毫米）相比，只有IGFBP - 1、IGFBP - 2、IGFBP - 3和IGFBP - 6的表达有所降低。总之，IGFs、它们的受体和IGFBPs在所分析的卵泡大小类别中表现出mRNA水平的不同表达。
