Zhang Ting, Mo Xian-Ming
Sichuan University, Chengdu, China.
Yi Chuan. 2013 Apr;35(4):441-8. doi: 10.3724/sp.j.1005.2013.00441.
During zebrafish gastrulation, large cellular rearrangements create the formation of the three germ layers, ectoderm, mesoderm, and endoderm. This process includes three types of conserved morphogenetic movement: epiboly, involution, and convergent extension. Specially, the anterior movement of prechordal plate progenitors is essential for the location and differentiation of mesendoderm progenitors, and the pechordal plate progenitors'coherent migration is thought to be a good model to study the mechanism of cell movement in vivo. Gastrulation migration is known to be controlled by many signaling pathways such as Wnt/planar cell polarity signaling; however, the underlying molecular mechanism for cellular behavior remains unknown. At present, it is generally agree that cell adhesion and cytoskeletal rearrangement are critical factors during zebrafish gastrulation cell migration. In addition, the role of extraembryonic tissue (yolk syncytial layer) during gastrulation is concerned increasingly. Here, we described the essential factors for controlling cellular behaviors and highlighted the major issues and questions that require further investigation during zebra fish gastrular cell migration in order to provide a complete map containing all the factors for regulating gastrulation cell migration and their interactions on a cellular level.
在斑马鱼原肠胚形成过程中，大量细胞重排形成了外胚层、中胚层和内胚层这三个胚层。这个过程包括三种保守的形态发生运动：外包、内卷和汇聚延伸。特别地，前脊索板祖细胞的向前运动对于中内胚层祖细胞的定位和分化至关重要，并且脊索板祖细胞的协同迁移被认为是研究体内细胞运动机制的一个良好模型。已知原肠胚形成期的迁移受许多信号通路控制，如Wnt/平面细胞极性信号通路；然而，细胞行为的潜在分子机制仍然未知。目前，人们普遍认为细胞黏附和细胞骨架重排是斑马鱼原肠胚形成期细胞迁移过程中的关键因素。此外，胚外组织（卵黄合胞体层）在原肠胚形成过程中的作用越来越受到关注。在这里，我们描述了控制细胞行为的关键因素，并强调了斑马鱼原肠胚细胞迁移过程中需要进一步研究的主要问题，以便提供一张完整的图谱，包含调节原肠胚形成期细胞迁移的所有因素及其在细胞水平上的相互作用。
