Department of Orthopaedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou 213003, China.
Department of Orthopaedics, Nantong First People's Hospital, the Second Affiliated Hospital of Nantong University, Nantong 226001, China.
Joint Bone Spine. 2014 Jan;81(1):32-6. doi: 10.1016/j.jbspin.2013.03.017. Epub 2013 May 6.
To examine whether the apolipoprotein M (APOM) rs805297 G/T polymorphism is associated with risk of rheumatoid arthritis (RA) in a Chinese population.
We studied APOM rs805297 G/T gene polymorphism in 520 RA patients, and 520 controls in a Chinese population. Genotyping was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The blood plasma concentration of APOM was measured by an enzyme-linked immunosorbent assay in 84 RA patients and 84 controls.
When the APOM rs805297 G/T GG homozygote genotype was used as the reference group, the TT or GT/TT genotype was associated with an increased risk for RA (TT vs. GG, adjusted odds ratio 1.76, 95% CI 1.11-2.77, P=0.016; GT + TT vs. GG, adjusted odds ratio 1.30, 95% CI 1.02-1.67, P=0.037). The average concentration of APOM in plasma was significantly higher in RA patients compared to controls. Stratification analysis found a significantly increased risk for RA associated with the APOM rs805297 TT genotype among male patients, C-reactive protein (CRP)-positive patients, anticitrullinated protein/peptide antibodies (ACPA) - positive patients, rheumatoid factor (RF) - positive patients, patients with higher levels of the erythrocyte sedimentation rate (ESR), patients with higher DAS28 score and patients with higher functional class compared to the APOM rs805297 GG genotype.
These findings suggest that the functional single-nucleotide polymorphism APOM rs805297 G/T variant allele was associated with RA risk.
研究载脂蛋白 M（APOM）rs805297G/T 多态性与中国人群类风湿关节炎（RA）发病风险的关系。
采用基质辅助激光解吸电离飞行时间质谱法对 520 例 RA 患者和 520 例对照者的 APOM rs805297G/T 基因多态性进行检测，采用酶联免疫吸附试验检测 84 例 RA 患者和 84 例对照者的 APOM 血浆浓度。以 APOM rs805297GG 纯合子基因型为参照，比较 TT 或 GT/TT 基因型与 RA 发病风险的关系。
与 GG 基因型相比，TT 基因型或 GT/TT 基因型与 RA 发病风险增加相关（TT 基因型与 GG 基因型相比，调整后的比值比 1.76，95%可信区间 1.112.77，P=0.016；GT/TT 基因型与 GG 基因型相比，调整后的比值比 1.30，95%可信区间 1.021.67，P=0.037）。RA 患者的 APOM 血浆浓度显著高于对照组。分层分析发现，APOM rs805297TT 基因型与男性患者、C 反应蛋白（CRP）阳性患者、抗瓜氨酸化蛋白/肽抗体（ACPA）阳性患者、类风湿因子（RF）阳性患者、红细胞沉降率（ESR）较高患者、DAS28 评分较高患者和功能分级较高患者的 RA 发病风险增加相关，而与 APOM rs805297GG 基因型相比，其风险降低。
APOM rs805297G/T 变异等位基因与 RA 发病风险相关。
