ADRIA Normandie, Villers-Bocage, France.
Food Microbiol. 2013 Sep;35(2):99-107. doi: 10.1016/j.fm.2013.02.011. Epub 2013 Mar 1.
The microbial biodiversity and dynamics of king scallops meat and coral during cold storage (cold chain rupture: 1/3 storage time at 4 °C followed by 2/3 at 8 °C), was assessed by combining culture-dependant and -independent methods. Products were packaged as follows: aerobic, vacuum packed and 3 different CO2/N2 modified atmospheres and the impact of these conditions on the microbial communities was assessed. Results indicated that under air (current packaging condition), the dominant species corresponded to Brochothrix thermosphacta, Pseudomonas spp. and Shewanella spp. These species have regularly been associated in the literature with food (especially seafood), and product spoilage. Moellerella wisconsensis was the only species detected on VRBG medium, however, its impact on the food product is unclear. Packaging conditions influenced the ecosystem equilibrium and biodiversity. Except for day 8, the lowest counts for all studied flora were observed for modified atmosphere packaging (MAP) containing >80% CO2. Moreover, in these conditions, higher biodiversity by Temporal Temperature Gradient Gel Electrophoresis (TTGE) and the non-detection of specific flora (i.e. Pseudoalteromonas haloplanktis) were observed. At day 8, scallops packaged using these conditions were still acceptable from a sensorial point of view (odour), although the initial load of the king scallop was high (total psychrotrophic flora reached 5.5 log CFU/g).
采用培养依赖和非依赖方法相结合的方式，评估了在冷藏过程中（冷链破裂：1/3 的时间在 4°C 下储存，然后 2/3 的时间在 8°C 下储存），扇贝肉和珊瑚的微生物生物多样性和动态变化。产品采用以下方式进行包装：有氧包装、真空包装和 3 种不同的 CO2/N2 改良气氛包装，并评估了这些条件对微生物群落的影响。结果表明，在空气（当前的包装条件）下，优势物种为嗜热解糖梭菌、假单胞菌属和希瓦氏菌属。这些物种在文献中经常与食品（尤其是海鲜）和产品变质有关。莫拉氏菌属是唯一在 VRBG 培养基上检测到的物种，但它对食品产品的影响尚不清楚。包装条件影响生态系统平衡和生物多样性。除第 8 天外，所有研究菌群的计数在含有>80%CO2 的改良气氛包装（MAP）中最低。此外，在这些条件下，通过时温梯度凝胶电泳（TTGE）观察到更高的生物多样性，并且未检测到特定菌群（即盐单胞菌属）。在第 8 天，使用这些条件包装的扇贝从感官角度（气味）来看仍然可以接受，尽管扇贝的初始负荷很高（总嗜冷菌达到 5.5 log CFU/g）。
