Figueiredo A S, Bulla S C, Lunsford K V, Bulla C
Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
J Comp Pathol. 2013 Aug-Oct;149(2-3):298-302. doi: 10.1016/j.jcpa.2013.03.005. Epub 2013 May 8.
Thrombopoietin (THPO) is the major cytokine that regulates megakaryopoiesis and platelet production. Several human and murine studies have demonstrated that THPO is primarily synthesized in the liver, but the kidney, spleen and bone marrow are also sites of expression. The aim of this study was to determine THPO mRNA levels in a range of canine tissues by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Samples of bone marrow (n = 5), liver (n = 10), lung (n = 10), renal cortex (n = 10), renal medulla (n = 5) and spleen (n = 10) were obtained from 10 healthy, hound-cross dogs aged 6-8 months. The highest THPO mRNA levels were found in the liver, followed by the bone marrow, spleen, lung and kidney. There was a 13-fold difference in expression between liver and kidney. The bone marrow showed high levels of THPO mRNA in the absence of disease. The liver and bone marrow are likely to be the major sites of THPO production in the dog.
血小板生成素（THPO）是调节巨核细胞生成和血小板产生的主要细胞因子。多项人体和小鼠研究表明，THPO主要在肝脏中合成，但肾脏、脾脏和骨髓也是其表达部位。本研究的目的是通过逆转录定量聚合酶链反应（RT-qPCR）测定一系列犬类组织中THPO mRNA的水平。从10只6至8个月大的健康杂交猎犬中获取骨髓（n = 5）、肝脏（n = 10）、肺（n = 10）、肾皮质（n = 10）、肾髓质（n = 5）和脾脏（n = 10）的样本。THPO mRNA水平最高的是肝脏，其次是骨髓、脾脏、肺和肾脏。肝脏和肾脏之间的表达差异达13倍。在无疾病的情况下，骨髓显示出高水平的THPO mRNA。肝脏和骨髓可能是犬类中THPO产生的主要部位。
