South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Vodňany, Czech Republic.
Theriogenology. 2013 Jul 15;80(2):153-60. doi: 10.1016/j.theriogenology.2013.04.009. Epub 2013 May 10.
Spontaneous polyploidy has been frequently documented in various fish species. This process may lead to disruption in testicular development and function. In the present study, sperm morphology and motility, elements critical to male fertility, were characterized in the naturally occurring triploid (3n) and tetraploid (4n) European weatherfish, Misgurnus fossilis L. (Teleostei, Cobitidae) inhabiting the upper reaches of the Lužnice River in the Czech Republic. Sperm with smaller heads, shorter flagella, and a lower number of mitochondria was observed in 3n specimens compared with 4n, but no differences were observed in size of midpiece or ultrastructure of sperm. Similar to most teleosts, the European weatherfish spermatozoon lacked an acrosome and consisted of a head (containing DNA), a midpiece (containing mitochondria and proximal and distal centrioles), and a flagellum with 9 + 2 microtubular structure. Sperm velocity was significantly lower in individuals with 4n compared with 3n, whereas no difference in sperm motility was observed. The stepwise linear regression reported significant negative correlations between sperm velocity and length of sperm head (r = -0.92, P < 0.01). In conclusion, the data reported no effect of polyploidy on sperm ultrastructure and motility in the European weatherfish, although it can affect sperm velocity, possibly through differences in head size and the number of mitochondria, which provide ATP for sperm movement.
自发多倍体在各种鱼类中经常被记录。这个过程可能导致睾丸发育和功能的中断。在本研究中，形态和运动学特征，这两个对雄性生育能力至关重要的因素，在自然发生的三倍体（3n）和四倍体（4n）欧洲泥鳅（Misgurnus fossilis L.）中被描述。与 4n 相比，3n 标本的精子头部更小，鞭毛更短，线粒体数量更少，但中段和精子的超微结构大小没有差异。与大多数硬骨鱼一样，欧洲泥鳅的精子没有顶体，由头部（包含 DNA）、中段（包含线粒体和近端和远端中心粒）和鞭毛组成，具有 9 + 2 微管结构。与 3n 相比，4n 个体的精子速度显著降低，而精子活力没有差异。逐步线性回归报告了精子速度与精子头部长度之间存在显著的负相关（r = -0.92，P < 0.01）。总之，尽管多倍体可能会通过头部大小和线粒体数量的差异影响精子速度，从而为精子运动提供 ATP，但报告的数据显示多倍体对欧洲泥鳅的精子超微结构和活力没有影响。
