Pediatric Urology Research Center, Department of Pediatric Urology, Children's Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
J Pediatr Urol. 2013 Dec;9(6 Pt B):1084-92. doi: 10.1016/j.jpurol.2013.03.010. Epub 2013 May 9.
To examine the efficacy of nine antiapoptotic compounds in preventing the development of Adriamycin-induced fetal renal abnormalities or ameliorating the resultant renal damage in a rat model.
Thirty-three Sprague-Dawley rats were randomly divided into sham-control, Adriamycin and prevention groups. The prevention group was divided into 9 subgroups. The rats were time mated and experimental rats were injected with Adriamycin on gestational day 7-9. Sham-control rats were injected with saline on the same days. The preventive medications were administered to the prevention group from 7 days prior to mating to the end of pregnancy. Samples were prepared from fetuses for histological and biochemical analyses.
A total of 331 fetuses were recovered. There were no resorptions in the Deferoxamine, Amifostine and sham-control groups. Significant decrease of antioxidant activities was noted in the Adriamycin group compared to the sham-control group. In all prevention groups, antioxidant activities were significantly increased compared to the Adriamycin group. The highest rate of hydronephrosis was observed in the Adriamycin group (82%). The lowest rates of renal abnormalities were noted with Deferoxamine and Amifostine: 8% and 11%.
Oxidant injury plays a critical role in the development and progression of Adriamycin-induced fetal renal abnormalities. Some antiapoptotic medications, notably Deferoxamine and Amifostine, may have preventive and therapeutic potential in the management of fetal renal abnormalities.
研究 9 种抗凋亡化合物在预防阿霉素诱导的胎鼠肾异常发生或改善胎鼠肾损伤方面的疗效。
33 只 Sprague-Dawley 大鼠随机分为假手术对照组、阿霉素组和预防组。预防组又分为 9 个亚组。实验大鼠于妊娠第 7-9 天给予阿霉素，假手术对照组大鼠于相同天数给予生理盐水。预防组从配种前 7 天至妊娠结束给予预防性药物。从胎鼠中提取样本进行组织学和生化分析。
共回收 331 只胎鼠。在去铁胺、氨磷汀和假手术对照组中无胚胎吸收。与假手术对照组相比，阿霉素组的抗氧化活性显著降低。与阿霉素组相比，所有预防组的抗氧化活性均显著升高。阿霉素组的肾盂积水发生率最高（82%）。去铁胺和氨磷汀组的肾异常发生率最低，分别为 8%和 11%。
氧化应激损伤在阿霉素诱导的胎鼠肾异常的发生和进展中起关键作用。一些抗凋亡药物，特别是去铁胺和氨磷汀，可能在胎鼠肾异常的防治方面具有潜在的预防和治疗作用。
