Gasperowicz Malgorzata, Rai Anshita, Cross James C
Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Gene Expr Patterns. 2013 Oct;13(7):249-54. doi: 10.1016/j.gep.2013.04.006. Epub 2013 May 9.
Notch signaling is involved in cell lineage specification in many developing organs. In mice there are four known Notch receptor genes (Notch1-4) and five ligands genes (Dll1, 3, 4 and Jagged1 and 2). Notch2 is essential for development of placenta, an organ that mediates feto-maternal nutrient and gas exchange as well as maternal adaptations to pregnancy. However the role of other Notch receptors and ligands in placentation is not known. In order to gain better insight into the role of Notch signaling in mouse placenta we thoroughly analyzed mRNA expression of all Notch receptors and ligands in all trophoblast cell types from the embryonic day (E) 7.5 to E12.5, the period during which all of the substructures of the placenta develop. Here we show that Notch receptors and ligands are specifically and dynamically expressed in multiple cell layers of developing placenta. We found that the Notch2 receptor and Jagged1 and Jagged2 ligand genes are complementarily expressed in trophoblast cells of the chorion and its later derivatives in the labyrinth. Dll4 and Notch2 expression complement each other in the ectoplacental cone, while Dll1 and Notch2 are expressed in an ectoplacental cone derivative, the junctional zone. Moreover Dll4 and Notch2 are expressed at the ectoplacental cone-decidua interface at early stages of placentation. Additionally we show that Notch2 is dynamically expressed in all trophoblast giant cell subtypes, which is consistent with previous reports. Overall these expression pattern results suggest that Notch signaling may play several diverse roles during placenta development.
Notch信号通路参与许多发育器官中的细胞谱系特化。在小鼠中，已知有四个Notch受体基因（Notch1 - 4）和五个配体基因（Dll1、3、4以及Jagged1和2）。Notch2对于胎盘发育至关重要，胎盘是一个介导胎儿与母体营养和气体交换以及母体适应妊娠的器官。然而，其他Notch受体和配体在胎盘形成中的作用尚不清楚。为了更深入了解Notch信号通路在小鼠胎盘中的作用，我们全面分析了从胚胎第（E）7.5天到E12.5天所有滋养层细胞类型中所有Notch受体和配体的mRNA表达，这是胎盘所有亚结构发育的时期。在这里，我们表明Notch受体和配体在发育中的胎盘的多个细胞层中特异性且动态地表达。我们发现Notch2受体以及Jagged1和Jagged2配体基因在绒毛膜及其在迷路中的后续衍生物的滋养层细胞中互补表达。Dll4和Notch2在胎盘外锥体中相互补充表达，而Dll1和Notch2在胎盘外锥体衍生物即连接区中表达。此外，Dll4和Notch2在胎盘形成早期在胎盘外锥体 - 蜕膜界面表达。另外，我们表明Notch2在所有滋养层巨细胞亚型中动态表达，这与先前的报道一致。总体而言，这些表达模式结果表明Notch信号通路在胎盘发育过程中可能发挥多种不同作用。
