Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Oct;63(Pt 10):3807-3811. doi: 10.1099/ijs.0.051185-0. Epub 2013 May 10.
A Gram-reaction-negative, aerobic, motile by one polar flagellum, yellow-pigmented, rod-shaped bacterium, designated strain THG-B117(T), was isolated from soil of a cornus fruit field of Hoengseong province in South Korea and its taxonomic position was investigated by a polyphasic study. Strain THG-B117(T) grew well at 25-30 °C and at pH 6.0-8.0 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity, strain THG-B117(T) was shown to belong to the family Xanthomonadaceae and be related to Dyella japonica XD53(T) (98.7 % similarity), Dyella terrae JS14-6(T) (98.0 %), Dyella koreensis BB4(T) (96.9 %), Dyella soli JS12-10(T) (96.9 %) and Dyella thiooxydans ATSB10(T) (96.7 %). DNA-DNA hybridization experiments showed that DNA relatedness between strain THG-B117(T) and its phylogenetically closest neighbours was below 45.1 %. The G+C content of the genomic DNA of strain THG-B117(T) was 64.8 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unidentified aminolipids, unidentified aminophospholipids and unidentified phospholipids. Phenotypic and chemotaxonomic data (major ubiquinone was Q-8, and major fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and iso-C17 : 1ω9c) supported the affiliation of strain THG-B117(T) with the genus Dyella. The results of physiological and biochemical tests suggested that strain THG-B117(T) was different genotypically and phenotypically from recognized species of the genus Dyella, and represents a novel species of this genus. The name Dyella kyungheensis sp. nov. is proposed, with the type strain THG-B117(T) ( = KACC 16981(T) = JCM 18747(T)).
一株革兰氏反应阴性、需氧、单极鞭毛、黄色素、杆状细菌，命名为 THG-B117(T) 菌株，从韩国洪城省的一个山茱萸果实田的土壤中分离出来，并通过多相研究研究了其分类地位。THG-B117(T) 菌株在 25-30°C 和无 NaCl 的营养琼脂上 pH 值为 6.0-8.0 时生长良好。根据 16S rRNA 基因序列相似性，菌株 THG-B117(T) 属于黄单胞菌科，与 Dyella japonica XD53(T)（98.7%相似）、Dyella terrae JS14-6(T)（98.0%）、Dyella koreensis BB4(T)（96.9%）、Dyella soli JS12-10(T)（96.9%）和 Dyella thiooxydans ATSB10(T)（96.7%）关系最为密切。DNA-DNA 杂交实验表明，THG-B117(T) 菌株与其系统发育上最接近的近缘菌株之间的 DNA 相关性低于 45.1%。THG-B117(T) 菌株基因组 DNA 的 G+C 含量为 64.8mol%。主要极性脂为磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、未鉴定的胺脂、未鉴定的胺磷脂和未鉴定的磷脂。表型和化学分类学数据（主要醌为 Q-8，主要脂肪酸为 iso-C15:0、iso-C16:0、iso-C17:0 和 iso-C17:1ω9c）支持 THG-B117(T) 菌株与 Dyella 属的归属。生理生化试验结果表明，THG-B117(T) 菌株在基因型和表型上与公认的 Dyella 属种不同，代表该属的一个新种。建议将其命名为 Dyella kyungheensis sp. nov.，其模式菌株为 THG-B117(T)（=KACC 16981(T)=JCM 18747(T)）。
