Duan Hai-Jing, Han Ting, Wu Xiu-Li, Li Na, Chen Jing, Qin Lu-Ping
School of Pharmacy, Ningxia Medical University, Ningxia Research Center of Modern Hui Medicine Engineering and Techology, Yinchuan 750004, China.
Zhongguo Zhong Yao Za Zhi. 2013 Feb;38(3):325-30.
The research aimed to investigate the entophytic fungal community of Cynanchum Komarrovii, including the biodiversity in different organs and the correlations with ecological environment. Endophytic fungi with patent bioactivity were also rapidly screened.
PDA medium was used to isolate and purify the endophytic fungi from C. komarovii living in Shaanxi and Ningxia district, respectively. The strains were identified based on the morphological characteristics of the fungi and similarity of 5.8S gene and internal transcribed spacer (ITS) sequence. Pyriculaia oryzae model was applied to preliminarily screen the active fungi.
Ninety-four strains of endophytic fungi were isolated and identified to 9 species, 13 genera, 9 families and 6 orders, among them, 47 strains were from the plants living in Ningxia. And then, 5 of them were isolated from roots, 14 from branches, and 28 from leaves. They were identified belonging to 8 species, 9 genera, 5 families and 4 orders. Additionally, 47 strains were from the plants living in Shaanxi. 16 were isolated from the roots, 18 from branches, 13 from leaves. They were identified belonging to 5 species, 8 genera, 6 families and 4 orders. By preliminary screening, 18 strains of endophytes completely inhibited the germination of conidium, which showed a potential bioactivity for these fungi. Both N4 and S17 strains had stronger growth inhibition effect.
Endophytic fungi from desert plant C. komarovii have the feature of diversity. Different geographical environment and type of organizations lead to the significant difference on the quantity and the species composition. Most of fungi in Ningxia C. komarovii distribute in leaves. However, most of those in Shaanxi C. komarovii distribute in stems and leaves. It also indicated that endophytes from C. komarovii had a strong antifungal activity.
研究牛心朴子内生真菌群落，包括不同器官中的生物多样性及其与生态环境的相关性，并快速筛选具有潜在生物活性的内生真菌。
采用PDA培养基分别从陕西和宁夏地区的牛心朴子中分离纯化内生真菌。根据真菌的形态特征以及5.8S基因和内转录间隔区（ITS）序列的相似性对菌株进行鉴定。应用稻瘟病菌模型对活性真菌进行初步筛选。
共分离鉴定出94株内生真菌，分属于6目9科13属9种，其中47株来自宁夏地区的植株。其中，从根部分离到5株，从枝条分离到14株，从叶片分离到28株。它们分属于4目5科9属8种。另外，47株来自陕西地区的植株。从根部分离到16株，从枝条分离到18株，从叶片分离到13株。它们分属于4目6科8属5种。通过初步筛选，有18株内生菌能完全抑制分生孢子的萌发，显示出这些真菌具有潜在的生物活性。N4和S17菌株的生长抑制作用较强。
沙漠植物牛心朴子内生真菌具有多样性特征。不同的地理环境和组织类型导致内生真菌在数量和种类组成上存在显著差异。宁夏牛心朴子中的真菌大多分布在叶片中。然而，陕西牛心朴子中的真菌大多分布在茎和叶中。这也表明牛心朴子内生菌具有较强的抗真菌活性。
