Liang Zi-Ning, Zhu Hua, Lai Kai-Ping, Chen Long
Zhong Yao Cai. 2014 Apr;37(4):564-8.
To isolate and identify endophytic fungi from Brucea javanica, and to detect the antimicrobial activity of these strains.
Endophytic fungi were isolated by tissue inoculation culture and identified by conventional morphological characteristic method. Seven kinds of pathogenic fungi and three kinds of bacteria were used as targeting microbes to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods, respectively.
A total of 83 endophytic fungi strains were isolated from the root, stem, leaf and fruit of Brucea javanica. 34 strains were obtained from the stem, 32 strains were obtained from the leaf, 15 strains were isolated from the root and 2 strains came from the fruit. These 73 strains which had been identified attribute to 5 orders, 6 families and 12 genera. For the isolated strains, 14 strains had antifungal activities against at least one pathogenic fungi, 9 strains showed antibacterial activities against one or more bacteria. Especially, the strain YJ-17 which belonged to Phomopsis genus showed the best inhibitory effect on the targeting microbes.
The endophytic fungi from Brucea javanica show diversity and microbial inhibition activity, and are worthy for further study on plant disease controlling.
从鸦胆子中分离并鉴定内生真菌，并检测这些菌株的抗菌活性。
采用组织接种培养法分离内生真菌，通过传统形态学特征方法进行鉴定。分别以7种致病真菌和3种细菌为靶标微生物，采用平板对峙法和改良琼脂凝胶扩散法检测抑菌活性。
从鸦胆子的根、茎、叶和果实中总共分离出83株内生真菌菌株。从茎中获得34株，从叶中获得32株，从根中分离出15株，从果实中得到2株。已鉴定的这73株菌株隶属于5目、6科和12属。对于分离出的菌株，14株对至少一种致病真菌具有抗真菌活性，9株对一种或多种细菌表现出抗菌活性。特别是，属于拟茎点霉属的YJ-17菌株对靶标微生物表现出最佳抑制效果。
鸦胆子内生真菌具有多样性和抑菌活性，值得进一步研究用于植物病害防治。
