Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, CSS, Bl. G, 21949902 Rio de Janeiro, Brazil.
Mar Environ Res. 2013 Oct;91:34-40. doi: 10.1016/j.marenvres.2013.03.008. Epub 2013 Apr 23.
We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0 × 10(6) cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
我们报告了一种从海洋双壳类动物 Nodipecten nodosus （一种有商业价值的热带扇贝）消化腺中构建和表征的标准化 cDNA 文库。共测序了 288 个克隆，获得了 250 个独特序列。该 cDNA 文库显示出较小的序列冗余（2.3%）和高数量的重组（99.9%）和独立克隆（2.0×10(6) cfu），表明本研究中生成的 cDNA 文库是一种用于高效基因发现的有价值资源对于 N. nodosus 而言。通过基因本体论术语分配对 EST 功能注释表明，鉴定了可能涉及水产养殖和生态毒理学相关过程的序列，例如凋亡、生长、脂质代谢、繁殖、发育、应激反应和免疫。
