Hadachi Hirotaka, Saito Takashi
Applied Medical Engineering Science, Graduate School of Medicine, Yamaguchi University, Ube-shi, Yamaguchi, Japan.
Appl Opt. 2013 Apr 20;52(12):2694-705. doi: 10.1364/AO.52.002694.
Digital holographic microscopy (DHM) has been used to determine the morphology and shape of transparent objects. However, the obtained shape is often inaccurate depending on the object properties and the setup of the optical imaging system. To understand these effects, we developed a new DHM model on the basis of a hybrid pupil imaging and finite-difference time-domain method. To demonstrate this model, we compared the results of an experiment with those of a simulation using borosilicate glass microspheres and a mold with a linear step structure. The simulation and experimental results showed good agreement. We also showed how the curvature and refractive index of objects affect the accuracy of thickness measurements.
数字全息显微镜（DHM）已被用于确定透明物体的形态和形状。然而，根据物体特性和光学成像系统的设置，所获得的形状往往不准确。为了理解这些影响，我们基于混合光瞳成像和时域有限差分方法开发了一种新的DHM模型。为了验证该模型，我们将使用硼硅酸盐玻璃微球和具有线性台阶结构的模具进行的实验结果与模拟结果进行了比较。模拟和实验结果显示出良好的一致性。我们还展示了物体的曲率和折射率如何影响厚度测量的准确性。
