Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, the College of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi'an, Shaanxi 710069, PR China.
J Pharm Biomed Anal. 2013 Jul-Aug;81-82:202-9. doi: 10.1016/j.jpba.2013.03.028. Epub 2013 Apr 16.
Poria cocos epidermis is one of ancient traditional Chinese medicines (TCMs), which is usually used for the treatment of chronic kidney disease (CKD) for thousands of years in China. A metabonomic approach based on ultra performance liquid chromatography coupled with quadrupole time-of-flight high-sensitivity mass spectrometry (UPLC Q-TOF/HSMS) and a mass spectrometry(Elevated Energy) (MS(E)) data collection technique was developed to obtained a systematic view of the development and progression of CKD and biochemistry mechanism of therapeutic effects of P. cocos epidermis (Fu-Ling-Pi, FLP). By partial least squares-discriminate analysis, 19 metabolites were identified as potential biomarkers of CKD. Among the 19 biomarkers, 10 biomarkers including eicosapentaenoic acid, docosahexaenoic acid, lysoPC(20:4), lysoPC(18:2), lysoPC(15:0), lysoPE(20:0/0:0), indoxyl sulfate, hippuric acid, p-cresol sulfate and allantoin were reversed to the control level in FLP-treated groups. The study indicates that FLP treatment can ameliorate CKD by intervening in some dominating metabolic pathways, such as fatty acid metabolism, phospholipid metabolism, purine metabolism and tryptophan metabolism. This work was for the first time to investigate the FLP therapeutic effect based on metabonomics technology, which is a potentially powerful tool to study the TCMs.
茯苓皮是一种古老的中药（TCM），在中国已有数千年用于治疗慢性肾脏病（CKD）的历史。本研究采用基于超高效液相色谱-四极杆飞行时间高灵敏度质谱联用（UPLC-Q-TOF/HSMS）和质谱（Elevated Energy）（MS（E））数据采集技术的代谢组学方法，以获得对 CKD 发展和进展的系统认识以及茯苓皮（茯神皮，FLP）治疗作用的生物化学机制。通过偏最小二乘判别分析，鉴定出 19 种潜在的 CKD 生物标志物。在 19 种生物标志物中，10 种生物标志物（包括二十碳五烯酸、二十二碳六烯酸、溶血磷脂酰胆碱（20:4）、溶血磷脂酰胆碱（18:2）、溶血磷脂酰胆碱（15:0）、溶血磷脂酰乙醇胺（20:0/0:0）、吲哚硫酸、马尿酸、对甲酚硫酸酯和尿囊素）在 FLP 治疗组中恢复到对照水平。该研究表明，FLP 治疗可通过干预脂肪酸代谢、磷脂代谢、嘌呤代谢和色氨酸代谢等主导代谢途径来改善 CKD。本研究首次基于代谢组学技术研究了 FLP 的治疗作用，这是研究中药的一种很有前途的工具。
