Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China,
World J Microbiol Biotechnol. 2013 Nov;29(11):2105-14. doi: 10.1007/s11274-013-1375-4. Epub 2013 May 14.
This study compared the compositions and properties of lipopeptide products purified from liquid fermentation (LF) and solid-state fermentation (SSF) and transcription levels involved in lipopeptides under the two systems. Results of Fourier transform infrared spectroscopy and High performance liquid chromatography revealed no significant differences in the polarity and structure of the two lipopeptide products. A higher amino acid proportion was found for lipopeptide product in LF than in SSF. Lipopeptide product produced from LF had better emulsification and antagonistic activities than that from SSF. For SSF, the transcription accumulation levels of the lipopeptide synthetic genes srfAA and sfp were higher than for LF at the same stage. Transcripts for ituD and lpa-14 remained elevated for a longer period of time under SSF conditions, accounting for differences in the production and fermentation periods between SSF and LF. This is the first report that describes differences in lipopeptide product synthesis and molecular behaviors between LF and SSF systems.
本研究比较了液体发酵（LF）和固态发酵（SSF）中纯化的脂肽产物的组成和性质，以及这两种系统中涉及脂肽的转录水平。傅里叶变换红外光谱和高效液相色谱的结果表明，两种脂肽产物的极性和结构没有显著差异。LF 中脂肽产物的氨基酸比例高于 SSF。LF 产生的脂肽产物具有比 SSF 更好的乳化和拮抗活性。对于 SSF，在相同阶段，脂肽合成基因 srfAA 和 sfp 的转录积累水平高于 LF。ituD 和 lpa-14 的转录本在 SSF 条件下保持较长时间的升高，这解释了 SSF 和 LF 之间在生产和发酵周期上的差异。这是首次描述 LF 和 SSF 系统中脂肽产物合成和分子行为差异的报告。
