从早产的脐带华通氏胶中提取的干细胞具有神经胶质分化潜能。
Stem cells from umbilical cord Wharton's jelly from preterm birth have neuroglial differentiation potential.
机构信息
1Department of Obstetrics and Gynecology, University Hospital Bern, Switzerland.
出版信息
Reprod Sci. 2013 Dec;20(12):1455-64. doi: 10.1177/1933719113488443. Epub 2013 May 13.
Abstract
OBJECTIVE
The aim of the study is to determine the neuroglial differentiation potential of human Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) from preterm birth when compared to term delivery.
STUDY DESIGN
The WJ-MSCs from umbilical cords of preterm birth and term controls were isolated and induced into neural progenitors. The cells were analyzed for neuroglial markers by flow cytometry, real-time polymerase chain reaction, and immunocytochemistry.
RESULTS
Independent of gestational age, a subset of WJ-MSC displayed the neural progenitor cell markers Nestin and Musashi-1 and the mature neural markers microtubule-associated protein 2, glial fibrillary acidic protein, and myelin basic protein. Neuroglial induction of WJ-MSCs from term and preterm birth resulted in the enhanced transcription of Nestin and Musashi-1.
CONCLUSIONS
Undifferentiated WJ-MSCs from preterm birth express neuroglial markers and can be successfully induced into neural progenitors similar to term controls. Their potential use as cellular graft in neuroregenerative therapy for peripartum brain injury in preterm birth has to be tested.
摘要
目的
本研究旨在比较早产儿和足月产来源的华通氏胶间充质干细胞（WJ-MSCs）的神经胶质分化潜能。
研究设计
从早产和足月产婴儿的脐带中分离并诱导 WJ-MSC 成为神经前体细胞。通过流式细胞术、实时聚合酶链反应和免疫细胞化学分析细胞的神经胶质标志物。
结果
与胎龄无关，WJ-MSC 的一部分表达神经前体细胞标志物巢蛋白和 Musashi-1 以及成熟神经标志物微管相关蛋白 2、神经胶质纤维酸性蛋白和髓鞘碱性蛋白。来自足月和早产的 WJ-MSC 的神经胶质诱导导致巢蛋白和 Musashi-1 的转录增强。
结论
早产来源的未分化 WJ-MSC 表达神经胶质标志物，并且可以像足月对照一样成功诱导成为神经前体细胞。它们作为围产期脑损伤神经再生治疗中细胞移植物的潜在用途有待进一步研究。
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