Chen Shi-Lin, Yao Hui, Han Jian-Ping, Xin Tian-Yi, Pang Xiao-Hui, Shi Lin-Chun, Luo Kun, Song Jing-Yuan, Hou Dian-Yun, Shi Shang-Mei, Qian Zhong-Zhi
The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jan;38(2):141-8.
Since the research of molecular identification of Chinese Materia Medica (CMM) using DNA barcode is rapidly developing and popularizing, the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China. Based on the study on comprehensive samples, the DNA barcoding systems have been established to identify CMM, i.e. ITS2 as a core barcode and psbA-trnH as a complementary locus for identification of planta medica, and COI as a core barcode and ITS2 as a complementary locus for identification of animal medica. This article introduced the principle of molecular identification of CMM using DNA barcoding and its drafting instructions. Furthermore, its application perspective was discussed.
随着中药DNA条形码分子鉴定研究的迅速发展与推广,该方法的原理已被批准列入《中华人民共和国药典》增补本。基于对大量样品的研究,已建立了用于中药鉴定的DNA条形码系统,即以ITS2作为核心条形码、psbA-trnH作为植物类药材鉴定的补充位点,以及以COI作为核心条形码、ITS2作为动物类药材鉴定的补充位点。本文介绍了中药DNA条形码分子鉴定的原理及其起草说明,并对其应用前景进行了探讨。