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本文引用的文献

1
Haploinsufficiency of the genes encoding the tumor suppressor Pten predisposes zebrafish to hemangiosarcoma.肿瘤抑制基因 Pten 编码基因的杂合性缺失使斑马鱼易患血管肉瘤。
Dis Model Mech. 2012 Mar;5(2):241-7. doi: 10.1242/dmm.008326. Epub 2011 Nov 9.
2
von Hippel-Lindau tumor suppressor mutants faithfully model pathological hypoxia-driven angiogenesis and vascular retinopathies in zebrafish.von Hippel-Lindau 肿瘤抑制因子突变体在斑马鱼中忠实地模拟了病理性缺氧驱动的血管生成和血管性视网膜病变。
Dis Model Mech. 2010 May-Jun;3(5-6):343-53. doi: 10.1242/dmm.004036. Epub 2010 Mar 24.
3
Generation of clonal zebrafish lines and transplantable hepatic tumors.克隆斑马鱼系的建立和可移植性肝肿瘤。
Nat Protoc. 2010 Mar;5(3):383-94. doi: 10.1038/nprot.2010.8. Epub 2010 Feb 11.
4
Zebrafish tumor assays: the state of transplantation.斑马鱼肿瘤检测法：移植技术现状
Zebrafish. 2009 Dec;6(4):339-46. doi: 10.1089/zeb.2009.0607.
5
A restricted spectrum of NRAS mutations causes Noonan syndrome.NRAS 基因突变谱受到限制会导致努南综合征。
Nat Genet. 2010 Jan;42(1):27-9. doi: 10.1038/ng.497. Epub 2009 Dec 6.
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Mutations in CCBE1 cause generalized lymph vessel dysplasia in humans.CCBE1 基因突变导致人类全身性淋巴管发育不良。
Nat Genet. 2009 Dec;41(12):1272-4. doi: 10.1038/ng.484.
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Dissecting the roles of Raf- and PI3K-signalling pathways in melanoma formation and progression in a zebrafish model.在斑马鱼模型中剖析Raf和PI3K信号通路在黑色素瘤形成和进展中的作用。
Dis Model Mech. 2009 Jul-Aug;2(7-8):399-411. doi: 10.1242/dmm.001149. Epub 2009 May 26.
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Expression of H-RASV12 in a zebrafish model of Costello syndrome causes cellular senescence in adult proliferating cells.在斑马鱼科斯特洛综合征模型中，H-RASV12的表达会导致成年增殖细胞发生细胞衰老。
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9
Efficient transfection of primary zebrafish fibroblasts by nucleofection.通过核转染高效转染原代斑马鱼成纤维细胞。
Cytotechnology. 2006 Jun;51(2):105-10. doi: 10.1007/s10616-006-9018-3. Epub 2006 Sep 21.
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Fibroblast growth factor 2-induced angiogenesis in zebrafish: the zebrafish yolk membrane (ZFYM) angiogenesis assay.成纤维细胞生长因子 2 诱导斑马鱼血管生成：斑马鱼卵黄膜（ZFYM）血管生成测定法。
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从斑马鱼胚胎和肿瘤中提取细胞系。

Deriving cell lines from zebrafish embryos and tumors.

机构信息

University Medical Center Utrecht , Utrecht, The Netherlands.

出版信息

Zebrafish. 2013 Sep;10(3):316-25. doi: 10.1089/zeb.2013.0866. Epub 2013 May 14.

DOI:10.1089/zeb.2013.0866
PMID:23672287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3760073/
Abstract

Over the last two decades the zebrafish has emerged as a powerful model organism in science. The experimental accessibility, the broad range of zebrafish mutants, and the highly conserved genetic and biochemical pathways between zebrafish and mammals lifted zebrafish to become one of the most attractive vertebrate models to study gene function and to model human diseases. Zebrafish cell lines are highly attractive to investigate cell biology and zebrafish cell lines complement the experimental tools that are available already. We established a straightforward method to culture cells from a single zebrafish embryo or a single tumor. Here we describe the generation of fibroblast-like cell lines from wild-type and ptenb(-/-) embryos and an endothelial-like cell line from a tumor of an adult ptena(+/-)ptenb(-/-) zebrafish. This protocol can easily be adapted to establish stable cell lines from any mutant or transgenic zebrafish line and the average time to obtain a pro-stable cell line is 3-5 months.

摘要

在过去的二十年中，斑马鱼已成为科学领域中一种强大的模式生物。斑马鱼具有实验可及性、广泛的斑马鱼突变体以及与哺乳动物高度保守的遗传和生化途径，这些特点使斑马鱼成为研究基因功能和模拟人类疾病的最具吸引力的脊椎动物模型之一。斑马鱼细胞系非常适合研究细胞生物学，并且斑马鱼细胞系补充了现有的实验工具。我们建立了一种从单个斑马鱼胚胎或单个肿瘤中培养细胞的简单方法。在这里，我们描述了从野生型和 ptenb(-/-)胚胎中生成成纤维样细胞系，以及从成年 ptena(+/-)ptenb(-/-)斑马鱼肿瘤中生成内皮样细胞系的方法。该方案可以轻松地适用于从任何突变体或转基因斑马鱼系建立稳定的细胞系，获得原代稳定细胞系的平均时间为 3-5 个月。