RNA 结合基序蛋白 3 在人脑星形细胞瘤中的差异表达。
Differential expression of the RNA-binding motif protein 3 in human astrocytoma.
机构信息
Department of Neurosurgery, First Affiliated Hospital of People's Liberation Army General Hospital, Beijing 100048, China.
出版信息
Chin Med J (Engl). 2013;126(10):1948-52.
BACKGROUND
The RNA-binding motif protein 3 (RBM3), which is transcriptionally induced by low temperature and hypoxia, has recently been found to be upregulated in human tumors. However, its expression status in human astrocytoma is not well defned. This article focuses on the differential expression of RBM3 in human astrocytomas of different grades and normal brain tissues.
METHODS
RBM3 was detected in astrocytomas and normal brain tissues by quantitative real-time PCR, immunohistochemistry, and Western blotting. Analysis of variance was performed on the data from quantitative real-time PCR. The Fisher's exact test was used to analyze the immunohistochemistry results. A P-value of less than 0.05 indicates a statistically significant difference.
RESULTS
On one hand, the mRNA expression levels of three X-chromosome-related RBM genes (RBMX, RBM3, and RBM10) were detected by quantitative real-time PCR. The results showed that there were no significant differences in RBMX and RBM10 mRNA expression levels in human astrocytomas of different grades and normal brain tissues. However, RBM3 mRNA expression levels were elevated in high-grade (World Health Organization (WHO) Grade III-IV) astrocytomas versus low-grade (WHO Grade I-II) astrocytomas (5.06 ± 0.66 vs. 1.60 ± 0.58; P < 0.05) or normal controls (5.06 ± 0.66 vs. 1.03 ± 0.22; P < 0.05) as determined by quantitative real-time PCR analysis. On the other hand, immunohistochemistry showed an increased RBM3 labeling index in astrocytomas of different grades and normal brain tissues (positive staining rate: astrocytoma Grade IV, 92.9%; astrocytoma Grade III, 81.8%; astrocytoma Grade I-II, 50%; normal brain tissues, 37.5%; high-grade astrocytoma versus normal brain tissues, P < 0.05; high-grade astrocytoma versus low-grade astrocytoma, P < 0.05). The higher protein levels of RBM3 were also validated in high-grade astrocytomas and low-grade astrocytomas compared with normal brain tissues by Western blotting.
CONCLUSIONS
These data suggest that the overexpression of RBM3 may serve as an important molecular mechanism underlying astrocytic carcinogenesis. Moreover, RBM3 may have proliferative and/or proto-oncogenic functions in human astrocytomas.
背景
RNA 结合基序蛋白 3（RBM3）在低温和缺氧条件下转录诱导，最近发现其在人类肿瘤中上调。然而，其在人类星形细胞瘤中的表达状态尚不清楚。本文主要关注 RBM3 在不同级别星形细胞瘤和正常脑组织中的差异表达。
方法
通过实时定量 PCR、免疫组织化学和 Western blot 检测星形细胞瘤和正常脑组织中的 RBM3。对实时定量 PCR 数据进行方差分析。采用 Fisher 确切检验分析免疫组化结果。P 值小于 0.05 表示具有统计学意义。
结果
一方面，通过实时定量 PCR 检测三种 X 染色体相关 RBM 基因（RBMX、RBM3 和 RBM10）的 mRNA 表达水平。结果显示，不同级别星形细胞瘤（世界卫生组织（WHO）分级 III-IV）和正常脑组织中 RBMX 和 RBM10mRNA 表达水平无显著差异。然而，与低级别（WHO 分级 I-II）星形细胞瘤（5.06±0.66 比 1.60±0.58；P<0.05）或正常对照组（5.06±0.66 比 1.03±0.22；P<0.05）相比，高级别（WHO 分级 III-IV）星形细胞瘤中 RBM3mRNA 表达水平升高。另一方面，免疫组织化学显示不同级别星形细胞瘤和正常脑组织中 RBM3 标记指数增加（阳性染色率：星形细胞瘤 IV 级，92.9%；星形细胞瘤 III 级，81.8%；星形细胞瘤 I-II 级，50%；正常脑组织，37.5%；高级别星形细胞瘤与正常脑组织相比，P<0.05；高级别星形细胞瘤与低级别星形细胞瘤相比，P<0.05）。Western blot 也验证了 RBM3 蛋白水平在高级别星形细胞瘤和低级别星形细胞瘤中均高于正常脑组织。
结论
这些数据表明，RBM3 的过表达可能是星形细胞癌变的重要分子机制。此外，RBM3 可能在人类星形细胞瘤中具有增殖和/或原癌基因功能。
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