血小板活化因子对人嗜酸性粒细胞的脱粒作用
[Degranulation of human eosinophilic granulocytes by the platelet activating factor].
作者信息
Kroegel C, Venge P, Matthys H, Barnes P J
机构信息
Department of Thoracic Medicine, Brompton Hospital, University of London, United Kingdom.
出版信息
Pneumologie. 1990 Feb;44 Suppl 1:234-5.
Incubation of purified human eosinophil granulocytes (greater than 93%) with the platelet activating factor (PAF), led at concentrations of 1 pM to 10 microM, to a non-cytotoxic and dose-dependent liberation of proteins both from the "small" and the "specific" granules. The mean EC50 for this PAF-induced effect was 1.47 nM (n = 9), with a maximum at 100 nM PAF. The degranulating effect of PAF can be inhibited competitively by the specific PAF-receptor antagonist, WEB 2086 (KB between 12 and 13 nM), and is Ca2(+)-dependent.
将纯化的人嗜酸性粒细胞（纯度大于93%）与血小板活化因子（PAF）一起孵育，在1皮摩尔至10微摩尔的浓度范围内，会导致从“小”颗粒和“特异性”颗粒中以非细胞毒性且剂量依赖性的方式释放蛋白质。PAF诱导这种效应的平均半数有效浓度（EC50）为1.47纳摩尔（n = 9），在100纳摩尔PAF时达到最大值。PAF的脱颗粒效应可被特异性PAF受体拮抗剂WEB 2086竞争性抑制（解离常数KB在12至13纳摩尔之间），且依赖于Ca2⁺ 。
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